Evaluation of tissue PCA3 expression in prostate cancer by RNA in situ hybridization- a correlative study with urine PCA3 and TMPRSS2-ERG

被引：26
|
作者：
Warrick, Joshua I. [1 ]
Tomlins, Scott A. [1 ,2 ,3 ,4 ]
Carskadon, Shannon L. [1 ,2 ]
Young, Allison M. [1 ]
Siddiqui, Javed [1 ,2 ]
Wei, John T. [4 ]
Chinnaiyan, Arul M. [1 ,2 ,3 ,4 ,5 ]
Kunju, Lakshmi P. [1 ,2 ,3 ]
Palanisamy, Nallasivam [1 ,2 ,3 ]
机构：
[1] Univ Michigan, Sch Med, Dept Pathol, Ann Arbor, MI 48104 USA
[2] Univ Michigan, Sch Med, Michigan Ctr Translat Pathol, Ann Arbor, MI USA
[3] Univ Michigan, Sch Med, Ctr Comprehens Canc, Ann Arbor, MI USA
[4] Univ Michigan, Sch Med, Dept Urol, Ann Arbor, MI USA
[5] Univ Michigan, Sch Med, Howard Hughes Med Inst, Ann Arbor, MI 48109 USA
来源：
MODERN PATHOLOGY | 2014年 / 27卷 / 04期
基金：
美国国家卫生研究院;
关键词：
PCA3; prostate cancer; RNA in situ hybridization; ANTIBODY-BASED DETECTION; ETS GENE FUSIONS; INTRAEPITHELIAL NEOPLASIA; ANTIGEN; REARRANGEMENT STATUS; TMPRSS2/ERG; CARCINOMA; DD3;
D O I：
10.1038/modpathol.2013.169
中图分类号：
R36 [病理学];
学科分类号：
100104 ;
摘要：
PCA3 is a prostate-specific non-coding RNA, with utility as a urine-based early detection biomarker. Here, we report the evaluation of tissue PCA3 expression by RNA in situ hybridization in a cohort of 41 mapped prostatectomy specimens. We compared tissue PCA3 expression with tissue level ERG expression and matched pre-prostatectomy urine PCA3 and TMPRSS2-ERG levels. Across 136 slides containing 138 foci of prostate cancer, PCA3 was expressed in 55% of cancer foci and 71% of high-grade prostatic intraepithelial neoplasia foci. Overall, the specificity of tissue PCA3 was >90% for prostate cancer and high-grade prostatic intraepithelial neoplasia combined. Tissue PCA3 cancer expression was not significantly associated with urine PCA3 expression. PCA3 and ERG positivity in cancer foci was positively associated (P<0.01). We report the first comprehensive assessment of PCA3 expression in prostatectomy specimens, and find limited correlation between tissue PCA3 and matched urine in prostate cancer.
引用
收藏
页码：609 / 620
页数：12
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