The cryo-EM method microcrystal electron diffraction (MicroED)

被引：167

作者：

Nannenga, Brent L. ^{[1
,2
]}

Gonen, Tamir ^{[3
,4
,5
]}

机构：

[1] Arizona State Univ, Sch Engn Matter Transport & Energy, Chem Engn, Tempe, AZ 85281 USA

[2] Arizona State Univ, Biodesign Inst, Ctr Appl Struct Discovery, Tempe, AZ 85281 USA

[3] Univ Calif Los Angeles, Howard Hughes Med Inst, Los Angeles, CA 90024 USA

[4] Univ Calif Los Angeles, David Geffen Sch Med, Dept Biol Chem, Los Angeles, CA 90095 USA

[5] Univ Calif Los Angeles, David Geffen Sch Med, Dept Physiol, Los Angeles, CA 90095 USA

来源：

NATURE METHODS | 2019年 / 16卷 / 05期

基金：

美国国家卫生研究院;

关键词：

INITIO STRUCTURE DETERMINATION; X-RAY LASERS; DATA-COLLECTION; ATOMIC-RESOLUTION; CRYSTAL-STRUCTURE; PROTEIN CRYSTALS; TOMOGRAPHY; MICROSCOPY; BETA; NANOCRYSTALS;

D O I：

10.1038/s41592-019-0395-x

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In 2013 we established a cryo-electron microscopy (cryo-EM) technique called microcrystal electron diffraction (MicroED). Since that time, data collection and analysis schemes have been fine-tuned, and structures for more than 40 different proteins, oligopeptides and organic molecules have been determined. Here we review the MicroED technique and place it in context with other structure-determination methods. We showcase example structures solved by MicroED and provide practical advice to prospective users.

引用

页码：369 / 379

页数：11

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