A method for transformation of Drosophila germline cells with a high-concentration exogenous DNA

被引：2

作者：

Shilova, I. E. ^{[1
]}

Omel'yanchuk, L. V. ^{[1
]}

机构：

[1] Russian Acad Sci, Inst Cytol & Genet, Novosibirsk 630090, Russia

来源：

RUSSIAN JOURNAL OF GENETICS | 2007年 / 43卷 / 01期

基金：

俄罗斯基础研究基金会;

关键词：

D O I：

10.1134/S1022795407010127

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A method for transformation of Drosophila germline cells without DNA capillary injections is proposed. The method involves a microneedle puncture of embryos and can be realized using simple equipment.

引用

页码：80 / 83

页数：4

共 6 条

[1]

Ashburner M., 2005, DROSOPHILA LAB HDB

[2]

KARESS RR, 1985, P ELEMENT MEDIATED G, P121

[3] ON THE NUMBER OF BLOBS PER ENTANGLEMENT IN SEMIDILUTE AND GOOD SOLVENT SOLUTION - MELT INFLUENCE [J].

RASPAUD, E ;

LAIREZ, D ;

ADAM, M .

MACROMOLECULES, 1995, 28 (04) :927-933

[4]

ROBERTSON HM, 1988, GENETICS, V118, P461

[5] TRANSPOSITION OF CLONED P ELEMENTS INTO DROSOPHILA GERM LINE CHROMOSOMES [J].

SPRADLING, AC ;

RUBIN, GM .

SCIENCE, 1982, 218 (4570) :341-347

[6] THE PRICKING METHOD - A NEW EFFICIENT TECHNIQUE FOR MECHANICALLY INTRODUCING FOREIGN DNA INTO THE NUCLEI OF CULTURE CELLS [J].

YAMAMOTO, F ;

FURUSAWA, M ;

FURUSAWA, I ;

OBINATA, M .

EXPERIMENTAL CELL RESEARCH, 1982, 142 (01) :79-84

← 1 →