Towards a natural classification of annulatascaceae-like taxa.: introducing five new genera and eighteen new species from freshwater

Annulatascaceae is an interesting and taxonomically confused family as some family members have been placed in different orders/families in Diaporthomycetidae. Although taxonomic changes have been carried out for some genera, many are questionable or their placements are unstable due to a lack of DNA sequence data. In this study, a survey of freshwater annulatascaceae-like species resulted in 23 new taxa, including five new genera (Aquidictyomyces, Fusoidigranularius, Longivarius, Neodiluviicola and Obliquiminima) and 18 new species in 12 genera, i.e. Annulatascus (A. chiangmaiensis, A. nakhonensis and A. songkhlaensis), Aquapteridospora (A. aquatica), Aquidictyomyces (A. appendiculatus), Cancellidium (C. thailandense), Conlarium (C. subglobosum), Dictyosporella (D. chiangmaiensis and D. ellipsoidea), Distoseptispora (D. fasciculata, D. saprophytica and D. songkhlaensis), Fluminicola (F. striata), Junewangia (J. thailandica), Obliquiminima (O. hyalina), Sporidesmiella (S. obovoidia) and Sporidesmium (S. appendiculatum and S. chiangmaiense). Annulatascus aquatorbae and A. nilensis are excluded from Annulatascus sensu stricto, and placed in two new genera Longivarius and Fusoidigranularius, respectively. Diluviicola capensis is a new geographical record in Thailand, and D. aquatica is transferred to a new genus Neodiluviicola as N. aquatica. Obliquiminima which is morphologically similar to Ayria is introduced as a new sexual genus in Cancellidiaceae. Dictyosporella chiangmaiensis is introduced as the second sexual morph in the genus. Fluminicola thailandensis is synonymized under F. saprophytica and Distoseptispora submersa is synonymized under D. tectonae. Strain HKUCC 3710 under the name Cateractispora recepticuli is rectified as the type strain of Cataractispora receptaculorum. We report a new geographical and habitat record of Acrodictys porosiseptata from freshwater in Thailand; a new geographical record of Conlarium aquaticum in China; and a new geographical record of Diluviicola capensis in Thailand. All taxa are identified based on morphology and phylogenetic analyses of a combined LSU, ITS, TEF and RPB2 DNA sequence data. An updated review for Annulatascaceae, as well as its sexual and asexual allies, is provided under each entry based on our new data.