Partial characterization of α-amylase in the salivary glands of Lygus hesperus and L-lineolaris

被引：41

作者：

Zeng, FR ^{[1
]}

Cohen, AC ^{[1
]}

机构：

[1] USDA ARS, Biol Control & Mass Rearing Res Unit, Mississippi State, MS 39762 USA

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 2000年 / 126卷 / 01期

关键词：

digestive enzymes; salivary glands; isoelectric points (pl); alpha-amylase inhibitors; pH optimum; Miridae;

D O I：

10.1016/S0305-0491(00)00176-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The alpha-amylases in the salivary glands of Lygus hesperus Knight and L. lineolaris (Palisot de Beauvois) were isolated and purified by ion exchange chromatography, and by isoelectric focusing, respectively. The alpha-amylase from L. hesperus had an isoelectric point (pI) of 6.25, and a pH optimum of 6.5. The specific activity of alpha-amylases in the salivary glands of L. hesperus was 1.2 U/mg/ml. The alpha-amylase from L. lineolaris had a pI of 6.54, and a pH optimum of 6.5. The specific activity of alpha-amylase from L. lineolaris was 1.7 U/mg/ml. The activity of alpha-amylase in both species was significantly inhibited by a-amylase inhibitor from wheat and also by EDTA and SDS. Sodium chloride enhanced alpha-amylase activity for both species. The enzyme characteristics and relative activities are discussed in the context of differences phytophagous versus zoophagous habits in these two congeneric species. Published by Elsevier Science Inc.

引用

页码：9 / 16

页数：8

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