Presence of mitogen-activated protein kinase in retinal Muller cells and its neuroprotective effect ischemia-reperfusion injury

被引：36

作者：

Akiyama, H ^{[1
]}

Nakazawa, T ^{[1
]}

Shimura, M ^{[1
]}

Tomita, H ^{[1
]}

Tamai, M ^{[1
]}

机构：

[1] Tohoku Univ, Sch Med, Dept Ophthalmol, Aoba Ku, Sendai, Miyagi 9808574, Japan

来源：

NEUROREPORT | 2002年 / 13卷 / 16期

关键词：

ischemia-reperfusion; neuroprotection; retinal ganglion cell; retinal Muller cells;

D O I：

10.1097/00001756-200211150-00022

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The purpose of this study was to determine whether the mitogen-activated protein kinase (MAPK) signaling pathway in the retina plays a neuroprotective role against ischemia-reperfusion injury. Western blot analysis showed that the MAPK activity was markedly increased within an hour after ischemia-reperfusion and subsequently decreased. Immunohistochemical studies revealed that MAPK was expressed mainly in the retinal Muller cells (RMCs). Pre-ischemic intravitreal administration of a MAPK inhibitor, U0126, increased the number of ganglion cell deaths induced by ischemia-reperfusion injury. We conclude that the MAPK activated in the RMCs protects ganglion cells against the ischemia-reperfusion injury through glia-neuronal interaction.

引用

页码：2103 / 2107

页数：5

共 25 条

[1] Cell survival promoted by the Ras-MAPK signaling pathway by transcription-dependent and -independent mechanisms [J].

Bonni, A ;

Brunet, A ;

West, AE ;

Datta, SR ;

Takasu, MA ;

Greenberg, ME .

SCIENCE, 1999, 286 (5443) :1358-1362

[2] Urocortin protects against ischemic and reperfusion injury via a MAPK-dependent pathway [J].

Brar, BK ;

Jonassen, AK ;

Stephanou, A ;

Santilli, G ;

Railson, J ;

Knight, RA ;

Yellon, DM ;

Latchman, DS .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (12) :8508-8514

[3] Serine/threonine protein kinases and apoptosis [J].

Cross, TG ;

Scheel-Toellner, D ;

Henriquez, NV ;

Deacon, E ;

Salmon, M ;

Lord, JM .

EXPERIMENTAL CELL RESEARCH, 2000, 256 (01) :34-41

[4] Regulation of retinal neurite growth by alterations in MAPK/ERK kinase (MEK) activity [J].

Dimitropoulou, A ;

Bixby, JL .

BRAIN RESEARCH, 2000, 858 (01) :205-214

[5] Possible mechanism for the decrease of mitochondrial aspartate aminotransferase activity in ischemic and hypoxic rat retinas [J].

Endo, S ;

Ishiguro, S ;

Tamai, M .

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 1999, 1450 (03) :385-396

[6] Circadian activation of bullfrog retinal mitogen-activated protein kinase associates with oscillator function [J].

Harada, Y ;

Sanada, K ;

Fukada, Y .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (47) :37078-37085

[7]

Inatani M, 2000, INVEST OPHTH VIS SCI, V41, P2748

[8] Expression of brain/kidney protein in Muller cells of rat retina following transient ischemia [J].

Ju, WK ;

Choi, SH ;

Kwon, JS ;

Kwon, OJ ;

Lee, MY ;

Oh, SJ ;

Moon, JI ;

Chun, MH .

NEUROSCIENCE LETTERS, 2000, 293 (01) :53-56

[9] Expression of CNTF in Muller cells of the rat retina after pressure-induced ischemia [J].

Ju, WK ;

Lee, MY ;

Hofmann, HD ;

Kirsch, M ;

Chun, MH .

NEUROREPORT, 1999, 10 (02) :419-422

[10] Nitric oxide synthase expression in ischemic rat retinas [J].

Kobayashi, M ;

Kuroiwa, T ;

Shimokawa, R ;

Okeda, R ;

Tokoro, T .

JAPANESE JOURNAL OF OPHTHALMOLOGY, 2000, 44 (03) :235-244

← 1 2 3 →