Zika virus crosses an in vitro human blood brain barrier model

被引：61

作者：

Alimonti, Judie B. ^{[1
]}

Ribecco-Lutkiewicz, Maria ^{[2
]}

Sodja, Caroline ^{[2
]}

Jezierski, Anna ^{[2
]}

Stanimirovic, Danica B. ^{[2
]}

Liu, Qing ^{[2
]}

Haqqani, Arsalan S. ^{[1
]}

Conlan, Wayne ^{[1
]}

Bani-Yaghoub, Mahmud ^{[2
]}

机构：

[1] Natl Res Council Canada, Human Hlth Therapeut Res Ctr, 100 Sussex Dr, Ottawa, ON, Canada

[2] Natl Res Council Canada, Human Hlth Therapeut Res Ctr, Bldg M54-1200,Montreal Rd, Ottawa, ON K1A 0R6, Canada

来源：

FLUIDS AND BARRIERS OF THE CNS | 2018年 / 15卷

关键词：

Zika virus; Brain endothelial cells; Blood-brain barrier model; iPSC; AXL; Neural progenitors; NEURAL PROGENITORS; CEREBRAL ORGANOIDS; ENDOTHELIAL-CELLS; INFECTION; AXL;

D O I：

10.1186/s12987-018-0100-y

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Zika virus (ZIKV) is a flavivirus that is highly neurotropic causing congenital abnormalities and neurological damage to the central nervous systems (CNS). In this study, we used a human induced pluripotent stem cell (iPSC)-derived blood brain barrier (BBB) model to demonstrate that ZIKV can infect brain endothelial cells (i-BECs) without compromising the BBB barrier integrity or permeability. Although no disruption to the BBB was observed post-infection, ZIKV particles were released on the abluminal side of the BBB model and infected underlying iPSC-derived neural progenitor cells (i-NPs). AXL, a putative ZIKV cellular entry receptor, was also highly expressed in ZIKV-susceptible i-BECand i-NPs. This iPSC-derived BBB model can help elucidate the mechanism by which ZIKV can infect BECs, cross the BBB and gain access to the CNS.

引用

页数：9

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