Zika virus crosses an in vitro human blood brain barrier model

被引:61
作者
Alimonti, Judie B. [1 ]
Ribecco-Lutkiewicz, Maria [2 ]
Sodja, Caroline [2 ]
Jezierski, Anna [2 ]
Stanimirovic, Danica B. [2 ]
Liu, Qing [2 ]
Haqqani, Arsalan S. [1 ]
Conlan, Wayne [1 ]
Bani-Yaghoub, Mahmud [2 ]
机构
[1] Natl Res Council Canada, Human Hlth Therapeut Res Ctr, 100 Sussex Dr, Ottawa, ON, Canada
[2] Natl Res Council Canada, Human Hlth Therapeut Res Ctr, Bldg M54-1200,Montreal Rd, Ottawa, ON K1A 0R6, Canada
来源
FLUIDS AND BARRIERS OF THE CNS | 2018年 / 15卷
关键词
Zika virus; Brain endothelial cells; Blood-brain barrier model; iPSC; AXL; Neural progenitors; NEURAL PROGENITORS; CEREBRAL ORGANOIDS; ENDOTHELIAL-CELLS; INFECTION; AXL;
D O I
10.1186/s12987-018-0100-y
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Zika virus (ZIKV) is a flavivirus that is highly neurotropic causing congenital abnormalities and neurological damage to the central nervous systems (CNS). In this study, we used a human induced pluripotent stem cell (iPSC)-derived blood brain barrier (BBB) model to demonstrate that ZIKV can infect brain endothelial cells (i-BECs) without compromising the BBB barrier integrity or permeability. Although no disruption to the BBB was observed post-infection, ZIKV particles were released on the abluminal side of the BBB model and infected underlying iPSC-derived neural progenitor cells (i-NPs). AXL, a putative ZIKV cellular entry receptor, was also highly expressed in ZIKV-susceptible i-BECand i-NPs. This iPSC-derived BBB model can help elucidate the mechanism by which ZIKV can infect BECs, cross the BBB and gain access to the CNS.
引用
收藏
页数:9
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