IRREGULAR POLLEN EXINE1 Is a Novel Factor in Anther Cuticle and Pollen Exine Formation

被引：88

作者：

Chen, Xiaoyang ^{[1
]}

Zhang, Hua ^{[3
,6
,7
]}

Sun, Huayue ^{[1
]}

Luo, Hongbing ^{[4
]}

Zhao, Li ^{[3
]}

Dong, Zhaobin ^{[1
]}

Yan, Shuangshuang ^{[2
]}

Zhao, Cheng ^{[5
]}

Liu, Renyi ^{[5
]}

Xu, Chunyan ^{[3
]}

Li, Song ^{[3
]}

Chen, Huabang ^{[3
]}

Jin, Weiwei ^{[1
]}

机构：

[1] China Agr Univ, MOE Key Lab Crop Heterosis & Utilizat, Natl Maize Improvement Ctr China, Beijing Key Lab Crop Genet Improvement, Beijing 100193, Peoples R China

[2] China Agr Univ, Dept Vegetable Sci, Beijing Key Lab Growth & Dev Regulat Protected Ve, Beijing 100193, Peoples R China

[3] Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Cell & Chromosome Engn, Beijing 100101, Peoples R China

[4] Hunan Agr Univ, Coll Agron, Southern Reg Collaborat Innovat Ctr Grain & Oil C, Changsha 410128, Hunan, Peoples R China

[5] Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Ctr Plant Stress Biol, Shanghai 201602, Peoples R China

[6] Univ Chinese Acad Sci, Beijing 100039, Peoples R China

[7] Shandong Acad Agr Sci, Maize Res Inst, Natl Engn Lab Wheat & Maize, Jinan 250100, Shandong, Peoples R China

来源：

PLANT PHYSIOLOGY | 2017年 / 173卷 / 01期

基金：

中国国家自然科学基金;

关键词：

TAPETUM-DEGENERATION-RETARDATION; FATTY-ACIDS; SPOROPOLLENIN SYNTHESIS; WAX BIOSYNTHESIS; WALL FORMATION; MALE-STERILE; GENE; EXPRESSION; HYDROXYLASE; REGULATOR;

D O I：

10.1104/pp.16.00629

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Anther cuticle and pollen exine are protective barriers for pollen development and fertilization. Despite that several regulators have been identified for anther cuticle and pollen exine development in rice (Oryza sativa) and Arabidopsis (Arabidopsis thaliana), few genes have been characterized in maize (Zea mays) and the underlying regulatory mechanism remains elusive. Here, we report a novel male-sterile mutant in maize, irregular pollen exine1 (ipe1), which exhibited a glossy outer anther surface, abnormal Ubisch bodies, and defective pollen exine. Using map-based cloning, the IPE1 gene was isolated as a putative glucose-methanolcholine oxidoreductase targeted to the endoplasmic reticulum. Transcripts of IPE1 were preferentially accumulated in the tapetum during the tetrad and early uninucleate microspore stage. A biochemical assay indicated that ipe1 anthers had altered constituents of wax and a significant reduction of cutin monomers and fatty acids. RNA sequencing data revealed that genes implicated in wax and flavonoid metabolism, fatty acid synthesis, and elongation were differentially expressed in ipe1 mutant anthers. In addition, the analysis of transfer DNA insertional lines of the orthologous gene in Arabidopsis suggested that IPE1 and their orthologs have a partially conserved function in male organ development. Our results showed that IPE1 participates in the putative oxidative pathway of C16/C18 omega-hydroxy fatty acids and controls anther cuticle and pollen exine development together with MALE STERILITY26 and MALE STERILITY45 in maize.

引用

页码：307 / 325

页数：19

共 67 条

[1] Molecular characterization of the CER1 gene of arabidopsis involved in epicuticular wax biosynthesis and pollen fertility [J].