Sequence analysis of single-copy genes in two wild olive subspecies: nucleotide diversity and potential use for testing admixture

被引:17
作者
Besnard, G. [1 ]
El Bakkali, A. [2 ]
机构
[1] UPS, CNRS, ENFA, EDB,UMR 5174, F-31062 Toulouse 9, France
[2] INRA, UR Ameliorat Plantes & Conservat Ressources Phyto, Meknes, Morocco
关键词
Australia; europaea; cuspidata; genetic admixture; invasive olive; Mediterranean basin; Red Sea coast; STRUCTURE; OLEA-EUROPAEA L; POPULATION-STRUCTURE; FLOW-CYTOMETRY; AFRICAN OLIVE; TREE; HYBRIDIZATION; AUSTRALIA; GENETICS; PATTERNS; DIFFERENTIATION;
D O I
10.1139/gen-2014-0001
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The wild olive distribution extends from the Mediterranean region to south Asia and Austral Africa. The species is also invasive, particularly in Australia. Here, we investigated the sequence variation at five nuclear single-copy genes in 41 native and invasive accessions of the Mediterranean and African olive subspecies. The nucleotide diversity was assessed and the phylogenetic relationships between alleles were depicted with haplotype networks. A Bayesian clustering method (STRUCTURE) was applied to identify the main gene pools. We found an average of 18.4 alleles per locus. Native Mediterranean and African olives only share one allele, which testifies for ancient admixture on the Red Sea hills. The presence of divergent alleles in the Mediterranean olive, as well as the identification of two main genetic clusters, suggests a complex origin with two highly differentiated gene pools from the eastern and western Mediterranean that recently admixed. In the invasive range, relatively high nucleotide diversity is observed as a consequence of the introduction of alleles from two subspecies. Our data confirm that four invasive individuals are early-generation hybrids. Finally, the utility of single-copy gene sequences in olive population genomic and phylogenetic studies is briefly discussed.
引用
收藏
页码:145 / 153
页数:9
相关论文
共 61 条
[1]   Comparative 454 pyrosequencing of transcripts from two olive genotypes during fruit development [J].
Alagna, Fiammetta ;
D'Agostino, Nunzio ;
Torchia, Laura ;
Servili, Maurizio ;
Rao, Rosa ;
Pietrella, Marco ;
Giuliano, Giovanni ;
Chiusano, Maria Luisa ;
Baldoni, Luciana ;
Perrotta, Gaetano .
BMC GENOMICS, 2009, 10 :399
[2]   Multiplexed shotgun genotyping for rapid and efficient genetic mapping [J].
Andolfatto, Peter ;
Davison, Dan ;
Erezyilmaz, Deniz ;
Hu, Tina T. ;
Mast, Joshua ;
Sunayama-Morita, Tomoko ;
Stern, David L. .
GENOME RESEARCH, 2011, 21 (04) :610-617
[3]   Olive genetic diversity assessed using amplified fragment length polymorphisms [J].
Angiolillo, A ;
Mencuccini, M ;
Baldoni, L .
THEORETICAL AND APPLIED GENETICS, 1999, 98 (3-4) :411-421
[4]  
[Anonymous], 1998, Molecular systematics of plants II: DNA sequencing, DOI DOI 10.1007/978-1-4615-5419-6_10
[5]   Median-joining networks for inferring intraspecific phylogenies [J].
Bandelt, HJ ;
Forster, P ;
Röhl, A .
MOLECULAR BIOLOGY AND EVOLUTION, 1999, 16 (01) :37-48
[6]   Genetic diversity and population structure of wild olives from the north-western Mediterranean assessed by SSR markers [J].
Belaj, Angjelina ;
Munoz-Diez, Concepcion ;
Baldoni, Luciana ;
Porceddu, Andrea ;
Barranco, Diego ;
Satovic, Zlatko .
ANNALS OF BOTANY, 2007, 100 (03) :449-458
[7]   Developing a core collection of olive (Olea europaea L.) based on molecular markers (DArTs, SSRs, SNPs) and agronomic traits [J].
Belaj, Angjelina ;
del Carmen Dominguez-Garcia, Maria ;
Gustavo Atienza, Sergio ;
Martin Urdiroz, Nieves ;
De la Rosa, Raul ;
Satovic, Zlatko ;
Martin, Antonio ;
Kilian, Andrzej ;
Trujillo, Isabel ;
Valpuesta, Victoriano ;
Del Rio, Carmen .
TREE GENETICS & GENOMES, 2012, 8 (02) :365-378
[8]  
Belkhir K., 2004, GENETIX 4.05, Logiciel Sous Windows TM Pour La Gntique Des Populations
[9]   Polyploidy in the olive complex (Olea europaea):: Evidence from flow cytometry and nuclear microsatellite analyses [J].
Besnard, G. ;
Garcia-Verdugo, C. ;
De Casas, R. Rubio ;
Treier, U. A. ;
Galland, N. ;
Vargas, P. .
ANNALS OF BOTANY, 2008, 101 (01) :25-30
[10]   Plastid and nuclear DNA polymorphism reveals historical processes of isolation and reticulation in the olive tree complex (Olea europaea) [J].
Besnard, G. ;
Rubio de Casas, R. ;
Vargas, P. .
JOURNAL OF BIOGEOGRAPHY, 2007, 34 (04) :736-752