Assistant deoxyribonucleic acid recycling with Zn2+ and molecular beacon for electrochemical detection of deoxyribonucleic acid via target-triggered assembly of mutated DNAzyme

被引：16
作者：
Qian, Yong [1 ]
Wang, Chunyan [1 ]
Gao, Fenglei [2 ]
机构：
[1] E China Inst Technol, Key Lab Radioact Geol & Explorat Technol Fundamen, Nanchang 344000, Peoples R China
[2] Xuzhou Med Coll, Sch Pharm, Jiangsu Key Lab Target Drug & Clin Applicat, Xuzhou 221004, Peoples R China
来源：
ANALYTICA CHIMICA ACTA | 2014年 / 845卷
基金：
中国国家自然科学基金;
关键词：
Deoxyribonucleic acid; Amplification; Electrochemical; DNAzyme; ROLLING CIRCLE AMPLIFICATION; CHAIN-REACTION AMPLIFICATION; DNA DETECTION; ENZYME-FREE; COLORIMETRIC DETECTION; GOLD NANOPARTICLES; NUCLEIC-ACIDS; LABEL-FREE; VIRUS DNA; NANOSTRUCTURES;
D O I：
10.1016/j.aca.2014.07.013
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A novel enzyme-free amplification strategy was designed for sensitive electrochemical detection of deoxyribonucleic acid (DNA) based on Zn2+ assistant DNA recycling via target-triggered assembly of mutated DNAzyme. A gold electrode was used to immobilize molecular beacon (MB) as the recognition probe and perform the amplification procedure. In the presence of target DNA, the hairpin probe 1 was opened, and the DNAzyme was liberated from the caged structure. The activated DNAzyme first hybridized and then cleaved the MB in the presence of cofactor Zn2+. After cleavage, the MB was cleaved into two pieces and the ferrocene (Fc) labeled piece dissociated from the gold electrode, thus obviously decreasing the Fc signal and forming a free DNAzyme strand. Finally, each target-induced activated DNAzyme underwent many cycles to trigger the cleavage of many MB substrates. Therefore, the peak current of Fc dramatically decreased to approximately zero. The strategy showed a detection limit at 35 fM levels, which was about 2 orders of magnitude lower than that of the conventional hybridization without Zn2+-based amplification. The Zn2+ assistant DNA recycling offers a versatile platform for DNA detection in a cost-effective manner, and has a promising application in clinical diagnosis. (C) 2014 Elsevier B.V. All rights reserved.
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页码：1 / 6
页数：6
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