Construction of an infectious Marek's disease virus bacterial artificial chromosome and characterization of protection induced in chickens

被引：17

作者：

Cui, Hong-yu ^{[1
,2
]}

Wang, Yun-feng ^{[1
]}

Shi, Xing-ming ^{[1
]}

An, Tong-qing ^{[1
]}

Tong, Guang-zhi ^{[1
,3
]}

Lan, De-song ^{[1
]}

He, Lai ^{[1
]}

Liu, Chang-jun ^{[1
]}

Wang, Mei ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Harbin Vet Res Inst, Natl Key Lab Vet Biotechnol, Div Avian Infect Dis, Harbin 150001, Peoples R China

[2] Hebei North Univ, Coll Anim Sci & Vet Med, Dept Anim Med, Zhang Jia Kou 075131, Peoples R China

[3] Chinese Acad Agr Sci, Shanghai Vet Res Inst, Shanghai 200232, Peoples R China

来源：

JOURNAL OF VIROLOGICAL METHODS | 2009年 / 156卷 / 1-2期

关键词：

Bacterial artificial chromosome (BAC); Marek's disease virus (MDV); ESCHERICHIA-COLI; REPLICATION-COMPETENT; SEROTYPE-1; MDV-1; DNA VACCINES; HERPESVIRUS; GENES; GROWTH; RECOMBINATION; GENERATION; GENOME;

D O I：

10.1016/j.jviromet.2008.10.021

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Marek's disease virus (MDV) is a highly oncogenic alphaherpesvirus that induces rapid-onset T-cell lymphoma in poultry. The complete genome of the avirulent vaccine strain MDV-814 was cloned as an infectious bacterial artificial chromosome (BAC) using an 8.8-kb fragment containing the self-designed selective marker guanosine phosphoriboxyl transferase. The recombinant virus MDV-814-BAC was generated by co-transfection of a BAC transfer vector and MDV-814 total DNA, and was purified by eight rounds of selective passaging. The infectivity of the BAC DNA clone was validated by MDV reconstitution from chicken embryo fibroblasts transfected with MDV-BAC DNA, which was extracted from electroporated Escherichia coli DH10B cells. In vitro, the BAC-derived virus had similar biological characteristics and growth kinetics as the wild-type parental and recombinant viruses, and chickens immunized with BAC derivatives by various delivery mechanisms acquired protection against virulent MDV challenge. Construction of this MDV-BAC may aid the development of recombinant vaccines-containing multiple antigens. (c) 2008 Elsevier B.V. All rights reserved.

引用

页码：66 / 72

页数：7

共 30 条

[1]

[Anonymous], 2012, Molecular Cloning: A Laboratory Manual

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