A Genetic Screen Based on in Vivo RNA Imaging Reveals Centrosome-Independent Mechanisms for Localizing gurken Transcripts in Drosophila

被引：12

作者：

Hayashi, Rippei ^{[1
]}

Wainwright, S. Mark ^{[1
]}

Liddell, Sophie J. ^{[1
]}

Pinchin, Sheena M. ^{[1
]}

Horswell, Stuart ^{[2
]}

Ish-Horowicz, David ^{[1
]}

机构：

[1] London Res Inst, Lab Dev Genet, London WC2A 3LY, England

[2] London Res Inst, Bioinformat Unit, London WC2A 3LY, England

来源：

G3-GENES GENOMES GENETICS | 2014年 / 4卷 / 04期

关键词：

RNA localization; whole-genome sequencing; mutant mapping; microtubules; oogenesis; EMBRYONIC AXIS SPECIFICATION; PRIMARY PIRNA BIOGENESIS; OSKAR MESSENGER-RNA; KINESIN-I; CELL-PROLIFERATION; CYTOPLASMIC DYNEIN; INDUCED MUTATIONS; OOCYTE POLARITY; TRANSPORT; OOGENESIS;

D O I：

10.1534/g3.114.010462

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have screened chromosome arm 3L for ethyl methanesulfonate-induced mutations that disrupt localization of fluorescently labeled gurken (grk) messenger (m)RNA, whose transport along microtubules establishes both major body axes of the developing Drosophila oocyte. Rapid identification of causative mutations by single-nucleotide polymorphism recombinational mapping and whole-genomic sequencing allowed us to define nine complementation groups affecting grk mRNA localization and other aspects of oogenesis, including alleles of elg1, scaf6, quemao, nudE, Tsc2/gigas, rasp, and Chd5/Wrb, and several null alleles of the armitage Piwi-pathway gene. Analysis of a newly induced kinesin light chain allele shows that kinesin motor activity is required for both efficient grk mRNA localization and oocyte centrosome integrity. We also show that initiation of the dorsoanterior localization of grk mRNA precedes centrosome localization, suggesting that microtubule self-organization contributes to breaking axial symmetry to generate a unique dorsoventral axis.

引用

页码：749 / 760

页数：12