Micropropagation of a difficult-to-root weeping mulberry (Morus alba var. Shidareguwa): A popular variety for ornamental purposes

被引:11
作者
Aroonpong, Piyathida [1 ]
Chang, Jer-Chia [1 ]
机构
[1] Natl Chung Hsing Univ, Dept Hort, Taichung 402, Taiwan
关键词
'Shidareguwa' mulberry; Micropropagation; Shoot tip; Shoot multiplication; Root induction; Acclimation; IN-VITRO MICROPROPAGATION; CLONAL PROPAGATION; NODAL EXPLANTS; BUD FORMATION; MATURE TREES; CULTURE; TISSUE; SEGMENTS; TAIWAN; GROWTH;
D O I
10.1016/j.scienta.2015.08.019
中图分类号
S6 [园艺];
学科分类号
0902 ;
摘要
'Shidareguwa' mulberry (Morus alba var. Shidareguwa) has a graceful weeping canopy that is commonly used in landscaping. However, it has a low rooting ability when conventionally propagated, and a feasible micropropagation system has not yet been reported. This study focused on establishing an in vitro method for plantlet production of 'Shidareguwa' mulberry. Explant types, media, and plant growth regulators (PGRs) were evaluated for their effects on shoot initial culture and multiplication, along with in vitro and ex vitro rooting and survival rate after acclimation and transfer into the field. Shoot tip explants had the highest survival percentage in initial culture. The largest shoot growths were cultured on Murashige and Skoog (MS) medium with 2% sucrose and 4.44 mu M 6-benzylaminopurine (BAP) for 20 days. The best shoot multiplication was achieved using MS medium with 3% sucrose and 4.44 mu M BAP for 30 days. The highest rooting induction (90%) in vitro was achieved using MS medium with 3% sucrose without auxins for 30 days. The well-rooted plantlets obtained in vitro had a 94% survival rate after acclimation. All young shoots from field-grown trees failed to root, but explants from in vitro regenerated shoots showed 100% root induction ex vitro when cultured in same media. These shoots dipped in 2000 ppm IBA exhibited the greatest rooting performance. Ex vitro plants exhibited greater rooting ability, efficiency, and survival after the first acclimation than in vitro plants. We present a novel and feasible method of plantlet production through in vitro propagation of shoot tips. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码:320 / 326
页数:7
相关论文
共 40 条
[1]  
Anirudh Thakur Anirudh Thakur, 2009, Haryana Journal of Horticultural Sciences, V38, P167
[2]  
[Anonymous], 2011, International Research Journal of Plant Science
[3]  
Balakrishnan V., 2009, BOT RES INT, V2, P42
[4]  
Baydar N., 2006, BIOTECHNOL EQUIP, P44
[5]   Rapid micropropagation of five cultivars of mulberry [J].
Bhau, BS ;
Wakhlu, AK .
BIOLOGIA PLANTARUM, 2003, 46 (03) :349-355
[6]   EARLY EVENTS OF MULTIPLE BUD FORMATION AND SHOOT DEVELOPMENT IN SOYBEAN EMBRYONIC AXES TREATED WITH THE CYTOKININ, 6-BENZYLAMINOPURINE [J].
BUISING, CM ;
SHOEMAKER, RC ;
BENBOW, RM .
AMERICAN JOURNAL OF BOTANY, 1994, 81 (11) :1435-1448
[7]  
Chang Jer-Chia, 2006, Journal of the Chinese Society for Horticultural Science, V52, P377
[8]   Phenotypic classification of mulberry (Morus) species in Taiwan using numerical taxonomic analysis through the characterization of vegetative traits and chilling requirements [J].
Chang, Lan-Yen ;
Li, Kuo-Tan ;
Yang, Wen-Ju ;
Chang, Jer-Chia ;
Chang, Ming-Wen .
SCIENTIA HORTICULTURAE, 2014, 176 :208-217
[9]  
Chitra DSV, 1999, SCI HORTIC-AMSTERDAM, V80, P289, DOI 10.1016/S0304-4238(98)00252-0
[10]   An efficient protocol for micropropagation of Melaleuca alternifolia Cheel [J].
de Oliveira, Yohana ;
Pinto, Fernanda ;
Lopes da Silva, Andre Luis ;
Guedes, Ivan ;
Biasi, Luiz Antonio ;
Quoirin, Marguerite .
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT, 2010, 46 (02) :192-197