Application of gold nanoparticles produced by laser ablation for immunochromatographic assay labeling

被引：26

作者：

Urusov, A. E. ^{[1
]}

Petrakova, A. V. ^{[1
]}

Kuzmin, P. G. ^{[2
]}

Zherdev, A. V. ^{[1
]}

Sveshnikov, P. G. ^{[3
]}

Shafeev, G. A. ^{[2
,4
]}

Dzantiev, B. B. ^{[1
]}

机构：

[1] Russian Acad Sci, AN Bach Inst Biochem, Biotechnol Res Ctr, Moscow 119071, Russia

[2] Russian Acad Sci, Wave Res Ctr, AM Prokhorov Gen Phys Inst, Moscow 119991, Russia

[3] Russian Natl Sci Ctr Mol Diagnost & Therapy, Moscow 117630, Russia

[4] Natl Res Nucl Univ MEPhI, Moscow Engn Phys Inst, Moscow 115409, Russia

来源：

ANALYTICAL BIOCHEMISTRY | 2015年 / 491卷

基金：

俄罗斯科学基金会;

关键词：

Laser ablation; Gold nanoparticles; Immunochromatography; Zearalenone; Mycotoxin; LINKED-IMMUNOSORBENT-ASSAY; COLLOIDAL GOLD; IMMUNOASSAY; ZEARALENONE; GENERATION; LIQUID; SIZE; FOOD;

D O I：

10.1016/j.ab.2015.08.031

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Nanodispersed gold is widely used as a marker in different analytical systems. For such purposes, it is usually obtained by the reduction of salts. This work studied the potential analytical applications of nanodispersed gold obtained by laser ablation because gold produced with this method has no chemical coating. The nanoparticles produced were characterized by transmission electron microscopy and spectrophotometry. The average size of the particles was 24.5 nm. Concentration dependences of antibody immobilization on ablative gold were obtained. With the use of antibody-conjugated nanoparticles, an immunochromatographic system was constructed for the detection of zearalenone mycotoxin. This immunoassay was characterized by a detection limit of 0.1 ng/ml antigen with an assay duration of only 15 min, which is on par with current test systems comprising nanodispersed gold obtained by chemical reduction. The simplicity of ablative dispersing makes this a prospective method for the labeling of various antibodies for analytical use. (C) 2015 Elsevier Inc. All rights reserved.

引用

页码：65 / 71

页数：7

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