Standard method for detecting upper respiratory carriage of Streptococcus pneumoniae: Updated recommendations from the World Health Organization Pneumococcal Carriage Working Group

被引：370

作者：

Satzke, Catherine ^{[1
,2
,3
]}

Turner, Paul ^{[4
,5
]}

Virolainen-Julkunen, Anni ^{[6
]}

Adrian, Peter V. ^{[7
]}

Antonio, Martin ^{[8
]}

Hare, Kim M. ^{[9
]}

Henao-Restrepo, Ana Maria ^{[10
]}

Leach, Amanda J. ^{[9
]}

Klugman, Keith P. ^{[11
,12
]}

Porter, Barbara D. ^{[1
]}

Sa-Leao, Raquel ^{[13
]}

Scott, J. Anthony ^{[14
,15
]}

Nohynek, Hanna ^{[16
]}

O'Brien, Katherine L. ^{[17
]}

机构：

[1] Royal Childrens Hosp, Murdoch Childrens Res Inst, Parkville, Vic 3052, Australia

[2] Royal Childrens Hosp, Murdoch Childrens Res Inst, Ctr Int Child Hlth, Parkville, Vic 3052, Australia

[3] Univ Melbourne, Dept Microbiol & Immunol, Parkville, Vic 3052, Australia

[4] Angkor Hosp Children, Dept Microbiol, Siem Reap, Cambodia

[5] Univ Oxford, Ctr Trop Med, Oxford, England

[6] Natl Inst Hlth & Welf, Dept Infect Dis Surveillance & Control, Helsinki, Finland

[7] Univ Witwatersrand, MRC Wits Resp & Meningeal Pathogens Res Unit, Johannesburg, South Africa

[8] MRC Unit, Banjul, Gambia

[9] Charles Darwin Univ, Menzies Sch Hlth Res, Child Hlth Div, Darwin, NT 0909, Australia

[10] WHO, Initiat Vaccine Res, CH-1211 Geneva, Switzerland

[11] Emory Univ, Rollins Sch Publ Hlth, Atlanta, GA 30322 USA

[12] Univ Witwatersrand, Resp & Meningeal Pathogens Res Unit, Johannesburg, South Africa

[13] Univ Nova Lisboa, Inst Tecnol Quim & Biol, Lab Mol Microbiol Human Pathogens, Oeiras, Portugal

[14] KEMRI Wellcome Trust Res Programme, Kilifi, Kenya

[15] London Sch Hyg & Trop Med, London WC1, England

[16] Natl Inst Hlth & Welf, Vaccine Programme Unit, Helsinki, Finland

[17] Johns Hopkins Bloomberg Sch Publ Hlth, Dept Int Hlth, Baltimore, MD USA

来源：

VACCINE | 2013年 / 32卷 / 01期

关键词：

Nasopharynx; Carriage; Colonization; Pneumococcus; CONJUGATE VACCINE; MULTIPLEX PCR; NASOPHARYNGEAL CARRIAGE; HAEMOPHILUS-INFLUENZAE; STAPHYLOCOCCUS-AUREUS; SAMPLING METHODS; NASAL CARRIAGE; POLYSACCHARIDE VACCINE; CLINICAL MICROBIOLOGY; SEROTYPE REPLACEMENT;

D O I：

10.1016/j.vaccine.2013.08.062

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

In 2003 the World Health Organization (WHO) convened a working group and published a set of standard methods for studies measuring nasopharyngeal carriage of Streptococcus pneumoniae (the pneumococcus). The working group recently reconvened under the auspices of the WHO and updated the consensus standard methods. These methods describe the collection, transport and storage of nasopharyngeal samples, as well as provide recommendations for the identification and serotyping of pneumococci using culture and non-culture based approaches. We outline the consensus position of the working group, the evidence supporting this position, areas worthy of future research, and the epidemiological role of carriage studies. Adherence to these methods will reduce variability in the conduct of pneumococcal carriage studies undertaken in the context of pneumococcal vaccine trials, implementation studies, and epidemiology studies more generally so variability in methodology does not confound the interpretation of study findings. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：165 / 179

页数：15

共 147 条

[1] Validation of nasopharyngeal sampling and culture techniques for detection of Streptococcus pneumoniae in children in Kenya [J].