Royal jelly stimulates bone formation: Physiologic and nutrigenomic studies with mice and cell lines

被引：47

作者：

Narita, Yukio

Nomura, Johji

Ohta, Shozo

Inoh, Yoshikazu

Suzuki, Kazu-Michi

Araki, Yoko

Okada, Shinji

Matsumoto, Ichiro

Isohama, Yoichiro

Abe, Keiko

Miyata, Takeshi

Mishima, Satoshi

机构：

[1] API Co Ltd, Nagaragawa Res Ctr, Gifu 5020071, Japan

[2] Kumamoto Univ, Fac Pharmaceut Sci, Kumamoto 8620973, Japan

[3] Univ Tokyo, Grad Sch Agr & Life Sci, Dept Appl Biol Chem, Tokyo 1138657, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2006年 / 70卷 / 10期

关键词：

royal jelly; MC3T3-E1; bone ash; type I procollagen;

D O I：

10.1271/bbb.60240

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Royal jelly (RJ) has diverse physiological and pharmacological functions. We observed its weak estrogenic activity in the previous study. RJ stimulated the proliferation of mouse osteoblast-like MC3T3-E1 cells at 0.1 mg/ml, and the effect was blocked by the specific estrogen receptor antagonist ICI 182,780. The addition of 0.1-1.0mg/ml RJ enhanced collagen production in culture medium. Oral administration of RJ to normal female mice for 9 weeks increased the ash content of their tibiae. DNA microarray analysis revealed significant changes in gene expression related to extracellular matrix formation when the femurs of mice fed RJ were analyzed. Quantitative reverse transcriptase-PCR (RTPCR) confirmed up-regulation of procollagen I alpha 1 gene expression. These data suggest that RJ as a whole or some of its individual components stimulates production of type I collagen and other activities for bone formation through action on osteoblasts.

引用

页码：2508 / 2514

页数：7