Propylene glycol releases calcium from mitochondrial stores in rat cerebrocortical synaptosomes

被引：2

作者：

Satoh, E ^{[1
]}

Murakami, K

Nishimura, M

机构：

[1] Obihiro Univ Agr & Vet Med, Dept Pathobiol Sci, Obihiro, Hokkaido 0808555, Japan

[2] Obihiro Univ Agr & Vet Med, Res Ctr Anim Hyg & Food Safety, Obihiro, Hokkaido 080, Japan

来源：

INTERNATIONAL JOURNAL OF NEUROSCIENCE | 2004年 / 114卷 / 09期

关键词：

cytosolic free calcium; fura-2; mitochondria; propylene glycol; rat; synaptosomes;

D O I：

10.1080/00207450490475553

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

In Ca2+-free medium, propylene glycol (PG) increases the cytosolic-free Ca2+ concentration ([Ca2+](i)) in rat cerebrocortical synaptosomes. In the current studies, the authors investigated the intracellular source of this Ca2+. PG (0.5-5% v/v) dose-dependently increased [Ca2+](i) in Ca2+-free medium. The increase in [Ca2+](i) was completely inhibited by pretreatment with the mitochondrial uncoupler, carbonylcyanide m-chlorophenylhydrazone (CCCP, 10 muM). The inhibitory effect of CCCP was dependent on the concentration and the pretreatment time. These results suggest that, in Ca2+-free medium, PG increases [Ca2+](i) in rat cerebrocortical synaptosomes by releasing Ca2+ front mitochondrial stores.

引用

页码：1111 / 1118

页数：8

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