Human single-donor composite skin substitutes based on collagen and polycaprolactone copolymer

被引：17

作者：

Dai, Niann-Tzyy ^{[1
]}

Yeh, Ming-Kung ^{[2
]}

Chiang, Chiao-Hsi ^{[3
]}

Chen, Ke-Chi ^{[4
]}

Liu, Tsung-Hsun ^{[5
]}

Feng, An-Chieh ^{[1
]}

Chao, Li-Lien ^{[6
]}

Shih, Chien-Ming ^{[6
]}

Sytwu, Huey-Kang ^{[7
]}

Chen, Shao-Liang ^{[1
]}

Chen, Tim-Mo ^{[1
]}

Adams, E. F. ^{[8
]}

机构：

[1] Tri Serv Gen Hosp, Div Plast & Reconstruct Surg, Natl Def Med Ctr, Dept Surg, Taipei, Taiwan

[2] Tri Serv Gen Hosp, Dept Clin Pharm, Taipei, Taiwan

[3] Natl Def Med Ctr, Sch Pharm, Taipei, Taiwan

[4] Tri Serv Gen Hosp, Div Pediat Surg, Dept Surg, Taipei, Taiwan

[5] Natl Def Med Ctr, Grad Inst Biol & Anat, Taipei, Taiwan

[6] Natl Def Med Ctr, Dept Trop Med & Parasitol, Taipei, Taiwan

[7] Natl Def Med Ctr, Dept Microbiol & Immunol, Taipei, Taiwan

[8] Aston Univ, Sch Pharm, Birmingham B4 7ET, W Midlands, England

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2009年 / 386卷 / 01期

关键词：

Skin substitute; Collagen; Poly(epsilon-caprolactone); Biocomposite; Keratinocyte; Fibroblast; Porosity; EXPRESSION; CLOSURE; TISSUE;

D O I：

10.1016/j.bbrc.2009.05.123

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The development and characterization of an enhanced composite skin substitute based on collagen and poly(epsilon-caprolactone) are reported. Considering the features of excellent biocompatibility, easy-manipulated property and exempt from cross-linking related toxicity observed in the 1:20 biocomposites, skin substitutes were developed by seeding human single-donor keratinocytes and fibroblasts alone on both sides of the 1:20 biocomposite to allow for separation of two cell types and preserving cell signals transmission via micro-pores with a porosity of 28.8 +/- 16.1 mu m. The bi-layered skin substitute exhibited both differentiated epidermis and fibrous dermis in vitro. Less Keratinocyte Growth Factor production was measured in the co-cultured skin model compared to fibroblast alone condition indicating a favorable microenvironment for epidermal homeostasis. Moreover, fast wound closure, epidermal differentiation, and abundant dermal collagen deposition were observed in composite skin in vivo. In summary, the beneficial characteristics of the new skin substitutes exploited the potential for pharmaceutical screening and clinical application. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：21 / 25

页数：5

共 13 条

[1]

ASMURSEN PD, 1986, EINFURUNG GRUNDLAGE, V1

[2] Host response to tissue engineered devices [J].

Babensee, JE ;

Anderson, JM ;

McIntire, LV ;

Mikos, AG .

ADVANCED DRUG DELIVERY REVIEWS, 1998, 33 (1-2) :111-139

[3] LIVING TISSUE FORMED INVITRO AND ACCEPTED AS SKIN-EQUIVALENT TISSUE OF FULL THICKNESS [J].

BELL, E ;

EHRLICH, HP ;

BUTTLE, DJ ;

NAKATSUJI, T .

SCIENCE, 1981, 211 (4486) :1052-1054

[4] SKIN ANATOMY AND ANTIGEN EXPRESSION AFTER BURN WOUND CLOSURE WITH COMPOSITE GRAFTS OF CULTURED SKIN CELLS AND BIOPOLYMERS [J].

BOYCE, ST ;

GREENHALGH, DG ;

KAGAN, RJ ;

HOUSINGER, T ;

SORRELL, JM ;

CHILDRESS, CP ;

RIEMAN, M ;

WARDEN, GD .

PLASTIC AND RECONSTRUCTIVE SURGERY, 1993, 91 (04) :632-641

[5]

CLARK RAF, 2000, PRINCIPLES TISSUE EN, P857

[6] Keratinocyte growth factor mRNA expression in periodontal ligament fibroblasts [J].

Dabelsteen, S ;

Wandall, HH ;

Gron, B ;

Dabelsteen, E .

EUROPEAN JOURNAL OF ORAL SCIENCES, 1997, 105 (06) :593-598

[7] A co-cultured skin model based on cell support membranes [J].

Dai, NT ;

Yeh, MK ;

Liu, DD ;

Adams, EF ;

Chiang, CH ;

Yen, CY ;

Shih, CM ;

Sytwu, HK ;

Chen, TM ;

Wang, HJ ;

Williamson, MR ;

Coombes, AGA .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2005, 329 (03) :905-908

[8] A bilayered living skin construct (APLIGRAF®) accelerates complete closure of hard-to-heal venous ulcers [J].

Falanga, V ;

Sabolinski, M .

WOUND REPAIR AND REGENERATION, 1999, 7 (04) :201-207

[9]

FUSENIG NE, 1991, EPITHELIAL MESENCHYM

[10] Extracellular matrix cell adhesion peptides: Functional applications in orthopedic materials [J].

Lebaron, RG ;

Athanasiou, KA .

TISSUE ENGINEERING, 2000, 6 (02) :85-103

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