Deep in vivo two-photon microscopy with a low cost custom built mode-locked 1060 nm fiber laser

被引:46
|
作者
Perillo, Evan P. [1 ]
McCracken, Justin E. [1 ]
Fernee, Daniel C. [1 ]
Goldak, John R. [1 ]
Medina, Flor A. [1 ]
Miller, David R. [1 ]
Yeh, Hsin-Chih [1 ]
Dunn, Andrew K. [1 ]
机构
[1] Univ Texas Austin, Dept Biomed Engn, 107 W Dean Keeton C0800, Austin, TX 78712 USA
来源
BIOMEDICAL OPTICS EXPRESS | 2016年 / 7卷 / 02期
基金
美国国家卫生研究院; 美国国家科学基金会;
关键词
DISSIPATIVE-SOLITON FIBER; PHOTONIC-CRYSTAL-FIBER; FLUORESCENT PROTEINS; OPTICAL-PROPERTIES; EMISSION-SPECTRA; EXCITATION; ABSORPTION; PULSES; TISSUE; BRAIN;
D O I
10.1364/BOE.7.000324
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Here we demonstrate that a mode-locked ytterbium fiber laser for two-photon fluorescence microscopy can be built for $13,000. The laser emits at a wavelength of 1060 nm with a usable average power of 1 W at a repetition rate of 40 MHz and a compressed pulse width of 81 fs at the sample. The laser is used to obtain deep in vivo two-color images of layer-V pyramidal neurons expressing YFP and vasculature labelled with Texas Red at depths up to 900 mu m. The sub-1 mu m features of dendritic spines can be resolved at a 200 mu m depth. (C) 2016 Optical Society of America
引用
收藏
页码:324 / 334
页数:11
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