Deep in vivo two-photon microscopy with a low cost custom built mode-locked 1060 nm fiber laser

被引：46

作者：

Perillo, Evan P. ^{[1
]}

McCracken, Justin E. ^{[1
]}

Fernee, Daniel C. ^{[1
]}

Goldak, John R. ^{[1
]}

Medina, Flor A. ^{[1
]}

Miller, David R. ^{[1
]}

Yeh, Hsin-Chih ^{[1
]}

Dunn, Andrew K. ^{[1
]}

机构：

[1] Univ Texas Austin, Dept Biomed Engn, 107 W Dean Keeton C0800, Austin, TX 78712 USA

来源：

BIOMEDICAL OPTICS EXPRESS | 2016年 / 7卷 / 02期

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

DISSIPATIVE-SOLITON FIBER; PHOTONIC-CRYSTAL-FIBER; FLUORESCENT PROTEINS; OPTICAL-PROPERTIES; EMISSION-SPECTRA; EXCITATION; ABSORPTION; PULSES; TISSUE; BRAIN;

D O I：

10.1364/BOE.7.000324

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Here we demonstrate that a mode-locked ytterbium fiber laser for two-photon fluorescence microscopy can be built for $13,000. The laser emits at a wavelength of 1060 nm with a usable average power of 1 W at a repetition rate of 40 MHz and a compressed pulse width of 81 fs at the sample. The laser is used to obtain deep in vivo two-color images of layer-V pyramidal neurons expressing YFP and vasculature labelled with Texas Red at depths up to 900 mu m. The sub-1 mu m features of dendritic spines can be resolved at a 200 mu m depth. (C) 2016 Optical Society of America

引用

页码：324 / 334

页数：11

共 35 条

[1] Grating-less, fiber-based oscillator that generates 25 nJ pulses at 80 MHz, compressible to 150 fs [J].

An, J. ;

Kim, D. ;

Dawson, J. W. ;

Messerly, M. J. ;

Barty, C. P. J. .

OPTICS LETTERS, 2007, 32 (14) :2010-2012

[2] High average and peak power femtosecond large-pitch photonic-crystal-fiber laser [J].

Baumgartl, Martin ;

Jansen, Florian ;

Stutzki, Fabian ;

Jauregui, Cesar ;

Ortac, Bulend ;

Limpert, Jens ;

Tuennermann, Andreas .

OPTICS LETTERS, 2011, 36 (02) :244-246

[3] Ultra-deep two-photon fluorescence excitation in turbid media [J].

Beaurepaire, E ;

Oheim, M ;

Mertz, J .

OPTICS COMMUNICATIONS, 2001, 188 (1-4) :25-29

[4] Two-photon fluorescence absorption and emission spectra of dyes relevant for cell imaging [J].

Bestvater, F ;

Spiess, E ;

Stobrawa, G ;

Hacker, M ;

Feurer, T ;

Porwol, T ;

Berchner-Pfannschmidt, U ;

Wotzlaw, C ;

Acker, H .

JOURNAL OF MICROSCOPY, 2002, 208 (02) :108-115

[5] In vivo local determination of tissue optical properties:: applications to human brain [J].

Bevilacqua, F ;

Piguet, D ;

Marquet, P ;

Gross, JD ;

Tromberg, BJ ;

Depeursinge, C .

APPLIED OPTICS, 1999, 38 (22) :4939-4950

[6] Properties of normal-dispersion femtosecond fiber lasers [J].

Chong, Andy ;

Renninger, William H. ;

Wise, Frank W. .

JOURNAL OF THE OPTICAL SOCIETY OF AMERICA B-OPTICAL PHYSICS, 2008, 25 (02) :140-148

[7] All-normal-dispersion femtosecond fiber laser with pulse energy above 20 nJ [J].

Chong, Andy ;

Renninger, William H. ;

Wise, Frank W. .

OPTICS LETTERS, 2007, 32 (16) :2408-2410

[8]

Denk W., 1990, NATURE, V248, P73

[9] Absolute Two-Photon Absorption Spectra and Two-Photon Brightness of Orange and Red Fluorescent Proteins [J].

Drobizhev, M. ;

Tillo, S. ;

Makarov, N. S. ;

Hughes, T. E. ;

Rebane, A. .

JOURNAL OF PHYSICAL CHEMISTRY B, 2009, 113 (04) :855-859

[10]

Drobizhev M, 2011, NAT METHODS, V8, P393, DOI [10.1038/NMETH.1596, 10.1038/nmeth.1596]

← 1 2 3 4 →