Curcumin inhibits telomerase activity through human telomerase reverse transcritpase in MCF-7 breast cancer cell line

被引：127
|
作者：
Ramachandran, C
Fonseca, HB
Jhabvala, P
Escalon, EA
Melnick, SJ
机构：
[1] Miami Childrens Hosp, Dept Pathol, Miami, FL 33155 USA
[2] Miami Childrens Hosp, Res Inst, Miami, FL 33155 USA
[3] Miami Childrens Hosp, Div Hematol & Oncol, Miami, FL 33155 USA
来源：
CANCER LETTERS | 2002年 / 184卷 / 01期
关键词：
curcumin; telomerase; human telomerase reverse transcriptase; telomerase RNA; c-myc; real-time reverse transcription-polymerase chain reaction;
D O I：
10.1016/S0304-3835(02)00192-1
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
The inhibitory effect of curcumin, the yellow-colored pigment from turmeric, on telomerase activity was analyzed in human mammary epithelial (MCF-10A) and breast cancer (MCF-7) cells. Telomerase activity in MCF-7 cells is 6.9-fold higher than that of human mammary epithelial cells. In MCF-7 cells, telomerase activity decreased with increasing concentrations of curcumin, inhibiting about 93.4% activity at 100 muM concentration. The inhibition of telomerase activity in MCF-7 cells may be due to down-regulation of hTERT expression. Increasing concentrations of curcumin caused a steady decrease in the level of hTERT mRNA in MCF-7 cells whereas the level of hTER and c-myc mRNAs remained the same. Our results suggest that curcumin inhibits telomerase activity by down-regulating hTERT expression in breast cancer cells and this down-regulation is not through the c-myc pathway. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.
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页码：1 / 6
页数：6
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