The use of peptones as medium additives for high-density perfusion cultures of animal cells

被引：0

作者：

Heidemann, R ^{[1
]}

Zhang, C ^{[1
]}

Qi, H ^{[1
]}

Rule, J ^{[1
]}

Rozales, C ^{[1
]}

Park, S ^{[1
]}

Chuppa, S ^{[1
]}

Ray, M ^{[1
]}

Michaels, J ^{[1
]}

Konstantinov, K ^{[1
]}

Naveh, D ^{[1
]}

机构：

[1] Bayer Corp, Berkeley, CA 94710 USA

来源：

ANIMAL CELL TECHNOLOGY: PRODUCTS FROM CELLS, CELLS AS PRODUCTS | 1999年

关键词：

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

This paper describes the test of several new vegetarian hydrolysates (peptones of soy, rice, wheat gluten etc.) as protein-free medium supplements for the production of a recombinant therapeutic protein. Multiple peptone-supplemented, continuous perfusion bioreactor experiments were conducted. Cell specific rates and product quality studies were obtained for the various peptones and compared with peptone-free medium. It was found that peptones confer a nutritional benefit, especially at low dilution rates, for a recombinant BHK cell line used in this investigation. The specific productivity increased 20-30% compared to the peptone-free controls. However, this benefit was also fully delivered by using fortified medium in place of the peptone-enriched media.

引用

页码：195 / 197

页数：3

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