Effect of pre-harvest treatments with salicylic and methyl salicylic acid on the chemical profile and activity of some phenylpropanoid pathway related enzymes in apple leaves

被引：33

作者：

Gacnik, Sasa ^{[1
]}

Veberic, Robert ^{[1
]}

Marinovic, Silvija ^{[2
]}

Halbwirth, Heidi ^{[2
]}

Mikulic-Petkovsek, Maja ^{[1
]}

机构：

[1] Univ Ljubljana, Dept Agron, Chair Fruit Viticulture & Vegetable Growing, Biotech Fac, Jamnikarjeva 101, SI-1000 Ljubljana, Slovenia

[2] Tech Univ Wien, Inst Chem Environm & Biosci Engn, Getreidemarkt 9, A-1060 Vienna, Austria

来源：

SCIENTIA HORTICULTURAE | 2021年 / 277卷

基金：

奥地利科学基金会;

关键词：

Salicylic acid; Methyl salicylic acid; Phenylpropanoid enzymes; Phenylalanine ammonia lyase (PAL); Phenolic compounds; Apple leaves; PHENYLALANINE AMMONIA-LYASE; PHENOLIC-COMPOUNDS; DIHYDROFLAVONOL; 4-REDUCTASE; BIOACTIVE COMPOUNDS; ANTIOXIDANT SYSTEM; FRUIT; BIOSYNTHESIS; ACCUMULATION; QUALITY; GRAPE;

D O I：

10.1016/j.scienta.2020.109794

中图分类号：

S6 [园艺];

学科分类号：

0902 ;

摘要：

The effects of single and multiple sprayings with salicylic acid (SA) and methyl-salicylic acid (MeSA) on the composition of phenolic compounds in apple leaves of the 'Topaz' cultivar were investigated by combined analysis of phenolic compounds by HPLC-MS and measurements of the activities of selected enzymes of the phenylpropanoid pathway (phenylalanine ammonia lyase (PAL), chalcone synthase (CHS)/chalcone isomerase (CHI), flavanone-38-hydroxylase (FHT) and dihydroflavonol 4-reductase (DFR)). The results showed that SA and MeSA in general can stimulate the activity of the above-mentioned enzymes, which led to an increase in certain proven antifungicidal phenolics, such as catechin, chlorogenic acid and phloridzin. MeSA increased the catechin content in apple leaves by up to 48 %, chlorogenic acid by up to 55 % and it also increased the content of phloridzin (1.7-fold higher content in comparison with the control). The content of some phenolic groups, such as hydroxycinnamic acid, flavanones and flavanols, increased with the number of sprayings with MeSA.

引用

页数：9

共 51 条

[1] Effect of pre- and postharvest salicylic on ethylene production, fungal decay acid treatment and overall quality of Selva strawberry fruit [J].