Methane metabolism in the archaeal phylum Bathyarchaeota revealed by genome-centric metagenomics

被引:591
作者
Evans, Paul N. [1 ]
Parks, Donovan H. [1 ]
Chadwick, Grayson L. [2 ]
Robbins, Steven J. [1 ]
Orphan, Victoria J. [2 ]
Golding, Suzanne D. [3 ]
Tyson, Gene W. [1 ,4 ]
机构
[1] Univ Queensland, Sch Chem & Mol Biosciences, Australian Ctr Ecogenom, St Lucia, Qld 4072, Australia
[2] CALTECH, Div Geol & Planetary Sci, Pasadena, CA 91125 USA
[3] Univ Queensland, Sch Earth Sci, St Lucia, Qld 4072, Australia
[4] Univ Queensland, Adv Water Management Ctr, St Lucia, Qld 4072, Australia
基金
澳大利亚研究理事会; 加拿大自然科学与工程研究理事会;
关键词
MISCELLANEOUS CRENARCHAEOTAL GROUP; COENZYME M REDUCTASE; ANAEROBIC OXIDATION; MARINE-SEDIMENTS; BENTHIC ARCHAEA; MCG ARCHAEA; IDENTIFICATION; ECOSYSTEMS; BACTERIA; INSIGHTS;
D O I
10.1126/science.aac7745
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Methanogenic and methanotrophic archaea play important roles in the global flux of methane. Culture-independent approaches are providing deeper insight into the diversity and evolution of methane-metabolizing microorganisms, but, until now, no compelling evidence has existed for methane metabolism in archaea outside the phylum Euryarchaeota. We performed metagenomic sequencing of a deep aquifer, recovering two near-complete genomes belonging to the archaeal phylum Bathyarchaeota (formerly known as the Miscellaneous Crenarchaeotal Group). These genomes contain divergent homologs of the genes necessary for methane metabolism, including those that encode the methyl-coenzyme M reductase (MCR) complex. Additional non-euryarchaeotal MCR-encoding genes identified in a range of environments suggest that unrecognized archaeal lineages may also contribute to global methane cycling. These findings indicate that methane metabolism arose before the last common ancestor of the Euryarchaeota and Bathyarchaeota.
引用
收藏
页码:434 / 438
页数:5
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