Determination of α-tocopherol in plasma by high performance liquid chromatography with fluorescence detection and stability of α-tocopherol under different conditions

被引：0

作者：

Rümelin, A ^{[1
]}

Humbert, T ^{[1
]}

Fauth, U ^{[1
]}

机构：

[1] Univ Mainz, Clin Anaesthesiol, D-6500 Mainz, Germany

来源：

ARZNEIMITTEL-FORSCHUNG-DRUG RESEARCH | 2004年 / 54卷 / 07期

关键词：

CAS; 30999-06-5; tocofersolan; alpha-tocopherol; determination in plasma; stability; vitamin E;

D O I：

暂无

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

In order to study the clinical effects of the antioxidant a-tocopherol (tocofersolan, CAS 30999-06-5) a simple, robust, sensitive and reliable high performance liquid chromatography (HPLC) method using fluorescence detection for the daily measurement of a-tocopherol concentration in plasma is described. Several methods of HPLC analysis using fluorescence techniques are published, however, investigations of the stability of alpha-tocopherol during sample preparation under different conditions are relevant in the routine measurements and have to date not been reported. Therefore a modified method, both sensitive and robust, qualified for the day-by-day measurement of a-tocopherol in plasma was established and the stability of a-tocopherol during sample preparation was analysed under different conditions. This method enables the complete separation of a-tocopherol from other compounds within 14 min. In addition, investigations on the stability of alpha-tocopherol during sample preparation showed the necessity of rapid sample preparation without delay. Prepared samples can be stored in liquid nitrogen for at least 56 days.

引用

页码：376 / 381

页数：6

共 30 条

[21] DETERMINATION OF ALPHA-TOCOPHEROL AND ALPHA-TOCOPHERYLQUINONE IN SMALL BIOLOGICAL SAMPLES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ELECTROCHEMICAL DETECTION [J].