Effect of metal ions on the enzymatic hydrolysis of hemp seed oil by lipase Candida sp 99-125

被引：14
作者：
Lu, Jike [1 ]
Wang, Pei [1 ]
Ke, Zhaodi [1 ]
Liu, Xin [1 ]
Kang, Qiaozhen [1 ]
Hao, Limin [2 ]
机构：
[1] Zhengzhou Univ, Sch Life Sci, Zhengzhou, Henan, Peoples R China
[2] Acad Mil Sci PLA China, Quartermaster Res Inst Engn & Technol, Beijing 100010, Peoples R China
来源：
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2018年 / 114卷
基金：
中国国家自然科学基金;
关键词：
Candida sp 99-25; Hemp seed oil; Metal ions; BOVINE SERUM-ALBUMIN; SOLVENT-FREE SYSTEM; BIODIESEL PRODUCTION; IMMOBILIZED LIPASE; WATER; ACID; PURIFICATION; STABILITY; IMPROVE;
D O I：
10.1016/j.ijbiomac.2018.03.168
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In order to study the effect of metal ions on the enzymatic hydrolysis of hemp seed oil by Candida sp. 99-125, the spectroscopy, stability and hydrolytic activity of the biocatalyst were investigated in presence of Ca2+, Mg2+,Fe2+, Fe3+, Cu2+, Sn2+, Pb2+, Zn2+ and Ba2+ metal ions, respectively. The UV spectroscopy showed that all the metal ions enhanced the absorbance but the decrease of fluorescence intensity was observed. All the metal ions could improve the lipase thermal stability except Cu2+ and Ba2+. Hydrolysis of hemp seed oil proved that Ca2+, Fe3+, Pb2+ and Ba2+ could significantly improve the hydrolytic rate, and metal ions could influence lipase selectivity. The study revealed that metal ions could improve lipase stability, hydrolysis activity in the hydrolytic process of hemp seed oil by Candida sp. 99-125. (C) 2018 Elsevier B.V. All rights reserved.
引用
收藏
页码：922 / 928
页数：7
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