Effect of metal ions on the enzymatic hydrolysis of hemp seed oil by lipase Candida sp 99-125

被引：14

作者：

Lu, Jike ^{[1
]}

Wang, Pei ^{[1
]}

Ke, Zhaodi ^{[1
]}

Liu, Xin ^{[1
]}

Kang, Qiaozhen ^{[1
]}

Hao, Limin ^{[2
]}

机构：

[1] Zhengzhou Univ, Sch Life Sci, Zhengzhou, Henan, Peoples R China

[2] Acad Mil Sci PLA China, Quartermaster Res Inst Engn & Technol, Beijing 100010, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2018年 / 114卷

基金：

中国国家自然科学基金;

关键词：

Candida sp 99-25; Hemp seed oil; Metal ions; BOVINE SERUM-ALBUMIN; SOLVENT-FREE SYSTEM; BIODIESEL PRODUCTION; IMMOBILIZED LIPASE; WATER; ACID; PURIFICATION; STABILITY; IMPROVE;

D O I：

10.1016/j.ijbiomac.2018.03.168

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In order to study the effect of metal ions on the enzymatic hydrolysis of hemp seed oil by Candida sp. 99-125, the spectroscopy, stability and hydrolytic activity of the biocatalyst were investigated in presence of Ca2+, Mg2+,Fe2+, Fe3+, Cu2+, Sn2+, Pb2+, Zn2+ and Ba2+ metal ions, respectively. The UV spectroscopy showed that all the metal ions enhanced the absorbance but the decrease of fluorescence intensity was observed. All the metal ions could improve the lipase thermal stability except Cu2+ and Ba2+. Hydrolysis of hemp seed oil proved that Ca2+, Fe3+, Pb2+ and Ba2+ could significantly improve the hydrolytic rate, and metal ions could influence lipase selectivity. The study revealed that metal ions could improve lipase stability, hydrolysis activity in the hydrolytic process of hemp seed oil by Candida sp. 99-125. (C) 2018 Elsevier B.V. All rights reserved.

引用

页码：922 / 928

页数：7

共 36 条

[1] Acikel U., 2011, EFFECTS COMPOSITION
[2] Ca2+-CaM regulating viability of Candida guilliermondii under oxidative stress by acting on detergent resistant membrane proteins
An, Bang
Chen, Yong
Li, Boqiang
Qin, Guozheng
Tian, Shiping
[J]. JOURNAL OF PROTEOMICS, 2014, 109 : 38 - 49
[3] Luciferase protection against proteolytic degradation: A key for improving signal in nano-system biology
Ataei, Farangis
Hosseinkhani, Saman
Khajeh, Khosro
[J]. JOURNAL OF BIOTECHNOLOGY, 2009, 144 (02) : 83 - 88
[4] Lipase immobilization on hyroxypropyl methyl cellulose support and its applications for chemo-selective synthesis of β-amino ester compounds
Badgujar, Kirtikumar C.
Bhanage, Bhalchandra M.
[J]. PROCESS BIOCHEMISTRY, 2016, 51 (10) : 1420 - 1433
[5] Purification of Free DHA by Selective Esterification of Fatty Acids from Tuna Oil Catalyzed by Rhizopus oryzae Lipase
Bhandari, Kriti
Chaurasia, S. P.
Dalai, A. K.
Gupta, Alok
[J]. JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 2013, 90 (11) : 1637 - 1644
[6] Efficacy of dietary hempseed oil in patients with atopic dermatitis
Callaway, J
Schwab, U
Harvima, I
Halonen, P
Mykkänen, O
Hyvönen, P
Järvinen, T
[J]. JOURNAL OF DERMATOLOGICAL TREATMENT, 2005, 16 (02) : 87 - 94
[7] Effect of ultrasound pre-treatment of hemp (Cannabis sativa L.) seed on supercritical CO2 extraction of oil
Da Porto, C.
Natolino, A.
Decorti, D.
[J]. JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE, 2015, 52 (03): : 1748 - 1753
[8] Relationship between thermal inactivation and conformational change of Yarrowia lipolytica lipase and the effect of additives on enzyme stability
Fu, Dayan
Li, Cong
Lu, Jike
Rahman, Ata ur
Tan, Tianwei
[J]. JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 2010, 66 (1-2) : 136 - 141
[9] Concentration of eicosapentaenoic acid and docosahexaenoic acid from fish oil by hydrolysis and urea complexation
Gámez-Meza, N
Noriega-Rodríguez, JA
Medina-Juárez, LA
Ortega-García, J
Monroy-Rivera, J
Toro-Vázquez, FJ
García, HS
Angulo-Guerrero, O
[J]. FOOD RESEARCH INTERNATIONAL, 2003, 36 (07) : 721 - 727
[10] Bacterial lipases: an overview of production, purification and biochemical properties
Gupta, R
Gupta, N
Rathi, P
[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2004, 64 (06) : 763 - 781

← 1 2 3 4 →