Quantum Dot-Based OFF/ON Probe for Detection of Glutathione

被引：109

作者：

Banerjee, Subhash ^{[1
]}

Kar, Soumitra ^{[1
]}

Perez, J. Manuel ^{[1
,2
,3
]}

Santra, Swadeshmukul ^{[1
,2
,3
]}

机构：

[1] Univ Cent Florida, NanoSci Technol Ctr, Orlando, FL 32826 USA

[2] Univ Cent Florida, Dept Chem, Orlando, FL 32826 USA

[3] Univ Cent Florida, Biomol Sci Ctr, Orlando, FL 32826 USA

来源：

JOURNAL OF PHYSICAL CHEMISTRY C | 2009年 / 113卷 / 22期

基金：

美国国家科学基金会;

关键词：

IN-VIVO; SOLVOTHERMAL PROCESS; SELECTIVE DETECTION; ELLMANS REAGENT; SOLAR-CELLS; CDS; FLUORESCENT; NANORODS; PHOTOLUMINESCENCE; QUANTIFICATION;

D O I：

10.1021/jp9019574

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

A simple strategy for detection of glutathione (GSH) in physiologically relevant concentrations is reported by manipulating the electron transfer pathways of surface-modified quantum dots. The probe consisted of fluorescent CdS:Mn/ZnS core/shell quantum dots (Qdot) properly conjugated to dopamine through CS2-assisted zero-length covalent coupling. Qdots exhibited bright yellow-orange emission at similar to 592 nm, which could be attributed to the T-4(1) --> (6)A(1) transition of Mn2+ ions in Qdot core. The fluorescently dark Qdots (OFF state) was obtained by modifying the Qdot surface with an electron rich dopamine molecule. The fluorescence of the probe was restored (ON state) when the disulfide bond was cleaved by the reducing GSH.

引用

页码：9659 / 9663

页数：5

共 33 条

[1] The role of glutathione in cancer [J].