Characterization of an Enterobacter cloacae Strain Producing both KPC and NDM Carbapenemases by Whole-Genome Sequencing

被引：42
作者：
Wu, Wenjing [1 ]
Feng, Yu [1 ]
Carattoli, Alessandra [2 ]
Zong, Zhiyong [1 ]
机构：
[1] Sichuan Univ, West China Hosp, Ctr Infect Dis, Chengdu 610064, Peoples R China
[2] Ist Super Sanita, Dept Infect Parasit & Immune Mediated Dis, I-00161 Rome, Italy
来源：
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2015年 / 59卷 / 10期
基金：
中国国家自然科学基金;
关键词：
KLEBSIELLA-PNEUMONIAE; HOSPITAL SEWAGE; BETA-LACTAMASE; EMERGENCE; PLASMIDS; ISOLATE; GENES; ARMA;
D O I：
10.1128/AAC.01275-15
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A carbapenem-resistant Enterobacter cloacae strain, WCHECl-14653, causing a fatal bloodstream infection, was characterized by genome sequencing and conjugation experiments. The strain carried two carbapenemase genes, bla(NDM-1) and bla(KPC-2), on separate IncF plasmids. The coexistence of bla(NDM-1) and bla(KPC-2) conferred slightly higher-level carbapenem resistance compared with that of bla(NDM-1) or bla(KPC-2) alone, and the coexistence of two IncF plasmids may generate new platforms for spreading carbapenemase genes.
引用
收藏
页码：6625 / 6628
页数：4
相关论文
共 18 条
  • [1] SPAdes: A New Genome Assembly Algorithm and Its Applications to Single-Cell Sequencing
    Bankevich, Anton
    Nurk, Sergey
    Antipov, Dmitry
    Gurevich, Alexey A.
    Dvorkin, Mikhail
    Kulikov, Alexander S.
    Lesin, Valery M.
    Nikolenko, Sergey I.
    Son Pham
    Prjibelski, Andrey D.
    Pyshkin, Alexey V.
    Sirotkin, Alexander V.
    Vyahhi, Nikolay
    Tesler, Glenn
    Alekseyev, Max A.
    Pevzner, Pavel A.
    [J]. JOURNAL OF COMPUTATIONAL BIOLOGY, 2012, 19 (05) : 455 - 477
  • [2] Emergence of carbapenem-resistant Klebsiella species possessing the class A carbapenem-hydrolyzing KPC-2 and inhibitor-resistant TEM-30 β-lactamases in New York City
    Bradford, PA
    Bratu, S
    Urban, C
    Visalli, M
    Mariano, N
    Landman, D
    Rahal, JJ
    Brooks, S
    Cebular, S
    Quale, J
    [J]. CLINICAL INFECTIOUS DISEASES, 2004, 39 (01) : 55 - 60
  • [3] CLSI, 2013, M100S23 CLIN LAB STA
  • [4] Single-molecule sequencing to track plasmid diversity of hospital-associated carbapenemase-producing Enterobacteriaceae
    Conlan, Sean
    Thomas, Pamela J.
    Deming, Clayton
    Park, Morgan
    Lau, Anna F.
    Dekker, John P.
    Snitkin, Evan S.
    Clark, Tyson A.
    Khai Luong
    Song, Yi
    Tsai, Yu-Chih
    Boitano, Matthew
    Dayal, Jyoti
    Brooks, Shelise Y.
    Schmidt, Brian
    Young, Alice C.
    Thomas, James W.
    Bouffard, Gerard G.
    Blakesley, Robert W.
    Mullikin, James C.
    Korlach, Jonas
    Henderson, David K.
    Frank, Karen M.
    Palmore, Tara N.
    Segre, Julia A.
    [J]. SCIENCE TRANSLATIONAL MEDICINE, 2014, 6 (254)
  • [5] Molecular dissection of the evolution of carbapenem-resistant multilocus sequence type 258 Klebsiella pneumoniae
    DeLeo, Frank R.
    Chen, Liang
    Porcella, Stephen F.
    Martens, Craig A.
    Kobayashi, Scott D.
    Porter, Adeline R.
    Chavda, Kalyan D.
    Jacobs, Michael R.
    Mathema, Barun
    Olsen, Randall J.
    Bonomo, Robert A.
    Musser, James M.
    Kreiswirth, Barry N.
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2014, 111 (13) : 4988 - 4993
  • [6] Small mobile sequences in bacteria display diverse structure/function motifs
    Delihas, Nicholas
    [J]. MOLECULAR MICROBIOLOGY, 2008, 67 (03) : 475 - 481
  • [7] The pCoo plasmid of enterotoxigenic Escherzchia coli is a mosaic cointegrate
    Froehlich, B
    Parkhill, J
    Sanders, M
    Quail, MA
    Scott, JR
    [J]. JOURNAL OF BACTERIOLOGY, 2005, 187 (18) : 6509 - 6516
  • [8] Complete Sequences of Two Plasmids in a blaNDM-1-Positive Klebsiella oxytoca Isolate from Taiwan
    Huang, Tzu-Wen
    Wang, Jann-Tay
    Lauderdale, Tsai-Ling
    Liao, Tsai-Lien
    Lai, Jui-Fen
    Tan, Mei-Chen
    Lin, Ann-Chi
    Chen, Ying-Tsong
    Tsai, Shih-Feng
    Chang, Shan-Chwen
    [J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2013, 57 (08) : 4072 - 4076
  • [9] Emergence of Klebsiella pneumoniae isolate co-producing NDM-1 with KPC-2 from India
    Kumarasamy, Karthikeyan
    Kalyanasundaram, Aravindan
    [J]. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2012, 67 (01) : 243 - 244
  • [10] Rapid detection and identification of metallo-β-lactamase-encoding genes by multiplex real-time PCR assay and melt curve analysis
    Mendes, Rodrigo E.
    Kiyota, Katia A.
    Monteiro, Jussimara
    Castanheira, Mariana
    Andrade, Soraya S.
    Gales, Ana C.
    Pignatari, Antonio C. C.
    Tufik, Sergio
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2007, 45 (02) : 544 - 547
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