Structures of the stator complex that drives rotation of the bacterial flagellum

被引:134
作者
Deme, Justin C. [1 ,2 ]
Johnson, Steven [1 ]
Vickery, Owen [3 ,4 ,5 ]
Muellbauer, Amy [1 ]
Monkhouse, Holly [1 ]
Griffiths, Thomas [1 ]
James, Rory Hennell [1 ]
Berks, Ben C. [3 ]
Coulton, James W. [6 ,7 ]
Stansfeld, Phillip J. [3 ,4 ,5 ]
Lea, Susan M. [1 ,2 ]
机构
[1] Univ Oxford, Sir William Dunn Sch Pathol, Oxford, England
[2] Univ Oxford, Cent Oxford Struct Mol Imaging Ctr, Oxford, England
[3] Univ Oxford, Dept Biochem, Oxford, England
[4] Univ Warwick, Sch Life Sci, Coventry, W Midlands, England
[5] Univ Warwick, Dept Chem, Coventry, W Midlands, England
[6] McGill Univ, Dept Microbiol & Immunol, Montreal, PQ, Canada
[7] Univ Montreal, Dept Biochem & Med Mol, Montreal, PQ, Canada
基金
芬兰科学院; 英国工程与自然科学研究理事会; 英国生物技术与生命科学研究理事会; 英国医学研究理事会; 英国惠康基金; 加拿大健康研究院;
关键词
ESCHERICHIA-COLI; CONFORMATIONAL-CHANGE; PERIPLASMIC DOMAIN; TORQUE GENERATION; CHARGED RESIDUES; NA+-DRIVEN; H+-DRIVEN; CRYO-EM; MOTOR; PROTEIN;
D O I
10.1038/s41564-020-0788-8
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Using single-particle analysis cryogenic electron microscopy, the authors determine the structures of the bacterial flagellum stator complexes from three diverse bacteria. The bacterial flagellum is the prototypical protein nanomachine and comprises a rotating helical propeller attached to a membrane-embedded motor complex. The motor consists of a central rotor surrounded by stator units that couple ion flow across the cytoplasmic membrane to generate torque. Here, we present the structures of the stator complexes fromClostridium sporogenes,Bacillus subtilisandVibrio mimicus, allowing interpretation of the extensive body of data on stator mechanism. The structures reveal an unexpected asymmetric A(5)B(2)subunit assembly where the five A subunits enclose the two B subunits. Comparison to structures of other ion-driven motors indicates that this A(5)B(2)architecture is fundamental to bacterial systems that couple energy from ion flow to generate mechanical work at a distance and suggests that such events involve rotation in the motor structures.
引用
收藏
页码:1553 / +
页数:26
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