Instrument-free point-of-care molecular diagnosis of H1N1 based on microfluidic convective PCR

被引:49
作者
Qiu, Xianbo [1 ]
Zhang, Shiyin [2 ]
Xiang, Fei [1 ]
Wu, Di [1 ]
Guo, Meng [1 ]
Ge, Shengxiang [2 ]
Li, Ke [3 ]
Ye, Xiangzhong [3 ]
Xia, Ningshao [2 ]
Qian, Shizhi [4 ]
机构
[1] Beijing Univ Chem Technol, Inst Microfluid Chip Dev Biomed Engn, Coll Informat Sci & Technol, Beijing 100029, Peoples R China
[2] Xiamen Univ, Natl Inst Diagnost & Vaccine Dev Infect Dis, Xiamen 361005, Peoples R China
[3] Beijing Wantai Biol Pharm Enterprise Co Ltd, Beijing 102206, Peoples R China
[4] Old Dominion Univ, Inst Micro Nanotechnol, Norfolk, VA 23529 USA
基金
中国国家自然科学基金;
关键词
Convective polymerase chain reaction; (CPCR); Nucleic acid analysis; Pseudo-isothermal; Chemically-heated thermal processor; Dipstick assay; INFLUENZA-VIRUSES; REPLICATION; DEVICES; SYSTEM; HEALTH; CHIP;
D O I
10.1016/j.snb.2016.12.058
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A simple, convenient, easy-to-use, and low-cost platform for rapid molecular detection of H1N1 virus is designed and implemented based on convective polymerase chain reaction (CPCR) and dipstick assay at the point of care (POC) settings. A fully disposable chemically-heated thermal processor is developed to heat the capillary tube from the bottom at a fixed temperature for amplification without the need for electrical power. Amplification is achieved when reagents in the capillary tube are spontaneously and repeatedly transported through different temperature zones, which significantly shortens the amplification time by eliminating the need for controlled time-domain temperature cycling. A rapid one-step nucleic acid dipstick assay is adopted to facilitate the detection of amplification products with the eye instead of instrumentation. The utility of our POC diagnostic system was demonstrated by detecting H1N1 virus with sensitivity of 1.0 TCID50/mL within 35 min. The developed system is particularly desired for resource poor settings, for example, developing countries, outside centralized laboratories, doctor's offices and homes, to relieve the requirements for laboratory facilities and trained personnel. (C) 2016 Elsevier B.V. All rights reserved.
引用
收藏
页码:738 / 744
页数:7
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