Proteomics-based sequence analysis of plant gene expression - the chloroplast transcription apparatus

被引：19

作者：

Loschelder, H ^{[1
]}

Homann, A ^{[1
]}

Ogrzewalla, K ^{[1
]}

Link, G ^{[1
]}

机构：

[1] Ruhr Univ Bochum, D-44780 Bochum, Germany

来源：

PHYTOCHEMISTRY | 2004年 / 65卷 / 12期

关键词：

chloroplast transcription; mass spectrometry; protein kinase; RNA-binding proteins; sequence database searches;

D O I：

10.1016/j.phytochem.2004.04.034

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The chloroplast transcription apparatus has turned out to be more complex than anticipated, with core polypeptides surrounded by multiple accessory proteins of diverse, and in part unexpected, functions. At least two different RNA-binding proteins and several redox-responsive proteins are components of the major chloroplast RNA polymerase termed PEP-A. One of the key-regulatory factors has been identified as a Ser/Thr-specific protein kinase that is sensitive to SH group modification by glutathione and by this means is able to modulate transcription. The cloned plastid transcription kinase from mustard (Sinapis alba L.) has been assigned as a member of the (mostly nucleo-cytosolic) CK2 family and hence has been termed cpCK2. Despite its apparent role in mustard chloroplast transcription, until recently no data have been available for other plant species. Using the web database resources, we find evidence for an evolutionarily conserved role of this redox-sensitive plastid transcription factor. (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：1785 / 1793

页数：9

共 36 条

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