Detection of Leishmania infantum DNA in phlebotomine sand flies from an area where canine leishmaniosis is endemic in southern Italy

被引:37
作者
Latrofa, Maria Stefania [1 ]
Iatta, Roberta [1 ]
Dantas-Torres, Filipe [1 ,2 ]
Annoscia, Giada [1 ]
Gabrielli, Simona [3 ]
Pombi, Marco [3 ]
Gradoni, Luigi [4 ]
Otranto, Domenico [1 ]
机构
[1] Univ Bari, Dept Vet Med, Str Prov Casamassima Km 3, I-70010 Bari, Italy
[2] Fundacao Oswaldo Cruz, Aggeu Magalhaes Inst, Dept Immunol, Recife, PE, Brazil
[3] Sapienza Univ Rome, Dept Publ Hlth & Infect Dis, Rome, Italy
[4] Ist Super Sanita, Dept Infect Dis, Rome, Italy
关键词
Leishmania infantum; Phlebotomus spp; Sergentomyia minute; Real-time PCR; POLYMERASE-CHAIN-REACTION; REAL-TIME PCR; DIPTERA PSYCHODIDAE; SERGENTOMYIA-MINUTA; MOLECULAR-DETECTION; 1ST DETECTION; BLOOD MEALS; IDENTIFICATION; PERNICIOSUS; SANDFLIES;
D O I
10.1016/j.vetpar.2018.02.006
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Phlebotomine sand flies (Diptera, Psychodidae) are vectors of Leishmania spp., among which Leishmania infantum is recognized as the main agent of human and canine leishmaniosis (CanL) in the Mediterranean area. In this study, females of Phlebotomus spp. (P. perniciosus, P. neglectus and P. papatasi) and Sergentomyia minuta were collected in a dog shelter of southern Italy, where CanL is endemic, and examined for Leishmania DNA. In total, 32 out of 56 of Phlebotomus spp. insects (57.1%) were found positive for L. infantum DNA by quantitative PCR (qPCR), with a mean parasite load of 1.9 x 10(3) promastigotes/ml among 23 positive P. perniciosus and 2.1 x 10(3) promastigotes/ml among five positive P. neglectus. Four P. papatasi, a species known to be refractory to L. infantum development, were also found positive. Among 216 S. minuta specimens examined, 25 (11.6%) scored positive for Leishmania tarentolae by conventional nested PCR; two (16.7%) of them were also positive for lizard blood, which is in agreement with the feeding preference of this phlebotomine species. Nine S. minuta (4.2%) were positive for L. infantum by qPCR, with a mean parasite load of 1.62 x 10(2) promastigotes/ml. The detection of L. infantum DNA in S. minuta may suggest that this species could acquire the protozoan, occasionally feeding on infected dogs. Further investigations need to clarify the potential role that S. minuta may have in the transmission of L. infantum to receptive mammal hosts.
引用
收藏
页码:39 / 42
页数:4
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