Local database and the search program for proteomic analysis of sperm proteins in the ascidian Ciona intestinalis

被引：27

作者：

Hozumi, A

Satouh, Y

Ishibe, D

Kaizu, M

Konno, A

Ushimaru, Y

Toda, T

Inaba, K ^{[1
]}

机构：

[1] Univ Tsukuba, Shimoda Marine Res Ctr, Shizuoka 4150025, Japan

[2] Tokyo Metropolitan Inst Gerontol, Tokyo 1730015, Japan

[3] Nara Inst Sci & Technol, Nara 6300101, Japan

[4] Tohoku Univ, Grad Sch Sci, Asamushi Marine Biol Stn, Asamushi, Aomori 0393501, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2004年 / 319卷 / 04期

关键词：

sperm motility; two-dimensional gel electrophoresis; MALDI-TOF/MS; proteomics; Ciona intestinalis; MS database;

D O I：

10.1016/j.bbrc.2004.05.118

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Separation of proteins by two-dimensional electrophoresis and following mass spectrometry (MS) is now a conventional technique for proteomic analysis. For proteomic analysis of a certain tissue with a limited information of primary structures of proteins, we have developed an analytical system for peptide mass fingerprinting in gene products in the testis of the ascidian Ciona intestinalis. Ciona sperm proteins were separated by two-dimensional gel electrophoresis and the tryptic fragments were subjected to MALDI-TOF/MS. The mass pattern was searched against on-line databases but resulted in less identification of these proteins. We have constructed a MS database from Ciona testis ESTs and the genome draft sequence, along with a newly devised, perl-based search program PerMS for peptide mass fingerprinting. This system could identify more than 80% of Ciona sperm proteins, suggesting that it could be widely applied for proteomic analysis for a limited tissue with less genomic information. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：1241 / 1246

页数：6

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