The role of polysaccharide intercellular adhesin (PIA) in Staphylococcus epidermidis adhesion to host tissues and subsequent antibiotic tolerance

被引：21
作者：
Costa, A. R. [1 ]
Henriques, M. [1 ]
Oliveira, R. [1 ]
Azeredo, J. [1 ]
机构：
[1] Univ Minho, IBB, Ctr Biol Engn, P-4710057 Braga, Portugal
来源：
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES | 2009年 / 28卷 / 06期
关键词：
CATHETER-ASSOCIATED INFECTION; MOLECULAR-BASIS; BIOFILM; RESISTANCE; ADHERENCE; PATHOGENESIS; IDENTIFICATION; SURFACE; CELLS; ADHESIN/HEMAGGLUTININ;
D O I：
10.1007/s10096-008-0684-2
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
The aim of this study was to determine the role of polysaccharide intercellular adhesin (PIA) in Staphylococcus epidermidis adhesion to host tissues and subsequent antibiotic tolerance. The adherence of S. epidermidis 1457 and the mutant defective in PIA production (1457-M10) to urinary epithelium and endothelium was estimated by colony counting. Minimum bactericidal concentration and mean reduction of cellular activity (XTT) following antibiotic exposure was determined for planktonic and adhered bacteria. S. epidermidis 1457 adhered to a greater extent to both cells than the mutant strain. The adhered strains had a significantly higher antimicrobial tolerance than their planktonic counterparts. The mutant strain was, in general, the most susceptible to the antibiotics assayed. In conclusion, PIA may influence S. epidermidis adherence to host tissues and their antimicrobial susceptibility. Initial adhesion may be the main step for the acquisition of resistance in S. epidermidis.
引用
收藏
页码：623 / 629
页数：7
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