Circular dichroism spectroscopy has intrinsic limitations for protein secondary structure analysis

被引：51

作者：

Khrapunov, Sergei ^{[1
]}

机构：

[1] Albert Einstein Coll Med, Dept Biochem, Bronx, NY 10461 USA

来源：

ANALYTICAL BIOCHEMISTRY | 2009年 / 389卷 / 02期

关键词：

SPECTRA; BIOINFORMATICS;

D O I：

10.1016/j.ab.2009.03.036

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Secondary structure content (SSC) cannot be calculated accurately from circular dichroism (CD) spectra for the majority of proteins whose three-dimensional structures have been solved. "Reliable" SSC that is significantly different from random SSC can be calculated from CD spectra only for all-alpha proteins and all-P proteins with canonical beta-strand geometry. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：174 / 176

页数：3

共 15 条

[1] Using circular dichroism spectra to estimate protein secondary structure [J].

Greenfield, Norma J. .

NATURE PROTOCOLS, 2006, 1 (06) :2876-2890

[2] Determination of the folding of proteins as a function of denaturants, osmolytes or ligands using circular dichroism [J].

Greenfield, Norma J. .

NATURE PROTOCOLS, 2006, 1 (06) :2733-2741

[3] Solvational tuning of the unfolding, aggregation and amyloidogenesis of insulin [J].

Grudzielanek, S ;

Jansen, R ;

Winter, R .

JOURNAL OF MOLECULAR BIOLOGY, 2005, 351 (04) :879-894

[4] Bioinformatics analyses of circular dichroism protein reference databases [J].

Janes, RW .

BIOINFORMATICS, 2005, 21 (23) :4230-4238

[5] Solution Structure and Refolding of the Mycobacterium tuberculosis Pentapeptide Repeat Protein MfpA [J].

Khrapunov, Sergei ;

Cheng, Huiyong ;

Hegde, Subray ;

Blanchard, John ;

Brenowitz, Michael .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2008, 283 (52) :36290-36299

[6] STRUCTURAL PATTERNS IN GLOBULAR PROTEINS [J].

LEVITT, M ;

CHOTHIA, C .

NATURE, 1976, 261 (5561) :552-558

[7]

MANAVALAN P, 1983, NATURE, V305, P831, DOI 10.1038/305831a0

[8] Improved estimation of the secondary structures of proteins by vacuum-ultraviolet circular dichroism spectroscopy [J].

Matsuo, K ;

Yonehara, R ;

Gekko, K .

JOURNAL OF BIOCHEMISTRY, 2005, 138 (01) :79-88

[9] Estimation of protein secondary structure from circular dichroism spectra: Comparison of CONTIN, SELCON, and CDSSTR methods with an expanded reference set [J].

Sreerama, N ;

Woody, RW .

ANALYTICAL BIOCHEMISTRY, 2000, 287 (02) :252-260

[10]

Sreerama N, 1999, PROTEIN SCI, V8, P370

← 1 2 →