Microcystin-RR-induced apoptosis in tobacco BY-2 cells

被引：19
作者：
Yin, Liyan
Huang, Jiaquan
Li, Wei
Liu, Yongding [1 ]
机构：
[1] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
[2] Chinese Acad Sci, Bot Gardens, Wuhan 430072, Peoples R China
[3] Chinese Acad Agr Sci, Oil Crops Res Inst, Wuhan 430062, Peoples R China
来源：
TOXICON | 2006年 / 48卷 / 02期
关键词：
microcystin-RR; tobacco BY-2 cells; apoptosis; flow cytometry; transmission electron microscopy;
D O I：
10.1016/j.toxicon.2006.05.002
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
Our previous studies showed that microcystin-RR could induce oxidative damage in plant cells as they do with animal cells. However, whether microcystin can induce plant cell apoptosis is still unknown. In this study, the morphological changes of tobacco BY-2 suspension cells exposed to microcystin-RR were observed under light microscopy and transmission electron microscopy, and apoptosis was clearly distinguished by intense perinuclear chromatin margination, condensation of nuclear chromatin after 6d exposure of 50 mg/L (about 50 mu M) microcystin-RR. We also found that microcystin-RR can induce tobacco cell apoptosis in a dose- and time-dependent manner with flow cytometry analysis. Our study provides the first evidence that microcystins can induce plant cell apoptosis. (c) 2006 Elsevier Ltd. All rights reserved.
引用
收藏
页码：204 / 210
页数：7
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