Identification of a lytic Pseudomonas aeruginosa phage depolymerase and its anti-biofilm effect and bactericidal contribution to serum

被引：43

作者：

Mi, Liyuan ^{[1
,2
,3
,4
]}

Liu, Yannan ^{[5
,7
]}

Wang, Can ^{[5
]}

He, Tongtong ^{[6
]}

Gao, Shan ^{[1
,2
,3
,4
]}

Xing, Shaozhen ^{[6
]}

Huang, Yong ^{[6
]}

Fan, Hang ^{[6
]}

Zhang, Xianglilan ^{[6
]}

Yu, Wengong ^{[1
,2
,3
,4
]}

Mi, Zhiqiang ^{[6
]}

Tong, Yigang ^{[6
]}

Bai, Changqing ^{[5
]}

Han, Feng ^{[1
,2
,3
,4
]}

机构：

[1] Ocean Univ China, Key Lab Marine Drugs, Chinese Minist Educ, 5 Yushan Rd, Qingdao 266003, Peoples R China

[2] Ocean Univ China, Key Lab Glycosci & Glycotechnol Shandong Prov, 5 Yushan Rd, Qingdao 266003, Peoples R China

[3] Ocean Univ China, Sch Med & Pharm, 5 Yushan Rd, Qingdao 266003, Peoples R China

[4] Ocean Univ China, Lab Marine Drugs & Bioprod, Qingdao Natl Lab Marine Sci & Technol, 5 Yushan Rd, Qingdao 266003, Peoples R China

[5] 307th Hosp PLA, Dept Resp & Crit Care Med, 8 Dongda St, Beijing 100071, Peoples R China

[6] Beijing Inst Microbiol & Epidemiol, State Key Lab Pathogen & Biosecur, Beijing 100071, Peoples R China

[7] Peking Univ, Peoples Hosp, Dept Resp & Crit Care Med, Beijing 100044, Peoples R China

来源：

VIRUS GENES | 2019年 / 55卷 / 03期

基金：

北京市自然科学基金; 中国国家自然科学基金;

关键词：

Pseudomonas aeruginosa; Bacteriophage; Depolymerase; Biofilm; Exopolysaccharide; BACTERIOPHAGE; LIPOPOLYSACCHARIDE; ADAPTATION; COMPLEMENT; IMMUNITY;

D O I：

10.1007/s11262-019-01660-4

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Pseudomonas aeruginosa (P. aeruginosa) infection has imposed a great threat to patients with cystic fibrosis. With the emergence of multidrug-resistant P. aeruginosa, developing an alternative anti-microbial strategy is indispensable and more urgent than ever. In this study, a lytic P. aeruginosa phage was isolated from the sewage of a hospital, and one protein was predicted as the depolymerase-like protein by genomic sequence analysis, it includes two catalytic regions, the Pectate lyase_3 super family and Glycosyl hydrolase_28 super family. Further analysis demonstrated that recombinant depolymerase-like protein degraded P. aeruginosa exopolysaccharide and enhanced bactericidal activity mediated by serum in vitro. Additionally, this protein disrupted host bacterial biofilms. All of these results showed that the phage-derived depolymerase-like protein has the potential to be developed into an anti-microbial agent that targets P. aeruginosa.

引用

页码：394 / 405

页数：12

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