Development of a wheat-Aegilops searsii substitution line with positively affecting Chinese steamed bread quality

被引:11
作者
Du, Xuye [1 ]
Ma, Xin [2 ]
Min, Jingzhi [1 ]
Zhang, Xiaocun [3 ]
Jia, Zhenzhen [4 ]
机构
[1] Guizhou Normal Univ, Sch Life Sci, 116 Baoshan North St, Guiyang 550001, Guizhou, Peoples R China
[2] Shandong Agr Univ, Coll Agron, 61 Daizong St, Tai An 271018, Shandong, Peoples R China
[3] Shandong Agr Univ, Coll Food Sci & Engn, 61 Daizong St, Tai An 271018, Shandong, Peoples R China
[4] Guizhou Normal Univ, Management Off Sci Res, 116 Baoshan North St, Guiyang 550001, Guizhou, Peoples R China
关键词
Aegilops searsii; wheat; substitution line; processing quality; HMW GLUTENIN SUBUNITS; MAKING QUALITY; GENETIC-VARIATION; ALLELIC VARIATION; DOUGH PROPERTIES; PAN BREAD; PROTEIN; MACROPOLYMER; TAUSCHII;
D O I
10.1270/jsbbs.17044
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
A wheat-Aegilops searsii substitution line GL1402, in which chromosome 1B was substituted with 1S(s) from Ae. searsii, was developed and detected using SDS-PAGE and GISH. The SDS-PAGE analysis showed that the HMW-GS encoded by the Glu-B1 loci of Chinese Spring was replaced by the HMW-GS encoded by the Glu-1S(s) loci of Ae. searsii. Glutenin macropolymer (GMP) investigation showed that GL1402 had a much higher GMP content than Chinese Spring did. A dough quality comparison of GL1402 and Chinese Spring indicated that GL1402 showed a significantly higher protein content and middle peak time (MPT), and a smaller right peak slope (RPS). Quality tests of Chinese steamed bread (CSB) showed that the GL1402 also produced good steamed bread quality. These results suggested that the substitution line is a valuable breeding material for improving the wheat processing quality.
引用
收藏
页码:289 / 293
页数:5
相关论文
共 26 条
[1]  
[Anonymous], 2005, Approved methods of the AACC
[2]   Rapid method for determination of protein content in cereals and oilseeds: validation, measurement uncertainty and comparison with the Kjeldahl method [J].
Beljkas, Bojana ;
Matic, Jovana ;
Milovanovic, Ivan ;
Jovanov, Pavle ;
Misan, Aleksandra ;
Saric, Ljubisa .
ACCREDITATION AND QUALITY ASSURANCE, 2010, 15 (10) :555-561
[3]   Genome analysis of Thinopyrum intermedium and Thinopyrum ponticum using genomic in situ hybridization [J].
Chen, Q ;
Conner, RL ;
Laroche, A ;
Thomas, JB .
GENOME, 1998, 41 (04) :580-586
[4]   Glutenin macropolymer: a gel formed by glutenin particles [J].
Don, C ;
Lichtendonk, W ;
Plijter, JJ ;
Hamer, RJ .
JOURNAL OF CEREAL SCIENCE, 2003, 37 (01) :1-7
[5]  
FLAVELL RB, 1989, BIO-TECHNOL, V7, P1281
[6]   Rheological, antioxidative and sensory properties of dough and Mantou (steamed bread) enriched with lemon fiber [J].
Fu, Jhong-Tai ;
Chang, Yung-Ho ;
Shiau, Sy-Yu .
LWT-FOOD SCIENCE AND TECHNOLOGY, 2015, 61 (01) :56-62
[7]   A second 'overexpression' allele at the Glu-B1 high-molecular-weight glutenin locus of wheat: sequence characterisation and functional effects [J].
Gao, Xin ;
Appelbee, Marie J. ;
Mekuria, Genet T. ;
Chalmers, Kenneth J. ;
Mather, Diane E. .
THEORETICAL AND APPLIED GENETICS, 2012, 124 (02) :333-343
[8]   Development of an Aegilops longissima substitution line with improved bread-making quality [J].
Garg, Monika ;
Kumar, Rohit ;
Singh, Rajinder Pal ;
Tsujimoto, Hisashi .
JOURNAL OF CEREAL SCIENCE, 2014, 60 (02) :389-396
[9]   Biochemical, genetic, and molecular characterization of wheat glutenin and its component subunits [J].
Gianibelli, MC ;
Larroque, OR ;
MacRitchie, F ;
Wrigley, CW .
CEREAL CHEMISTRY, 2001, 78 (06) :635-646
[10]   Composition of HMW and LMW glutenin subunits and their effects on dough properties, pan bread, and noodle quality of Chinese bread wheats [J].
He, ZH ;
Liu, L ;
Xia, XC ;
Liu, JJ ;
Peña, RJ .
CEREAL CHEMISTRY, 2005, 82 (04) :345-350