Novel mutations conferring resistance to kanamycin in Mycobacterium tuberculosis clinical isolates from Northern India

被引：10

作者：

Kaur, Simerpreet ^{[1
]}

Rana, Vibhuti ^{[1
]}

Singh, Pooja ^{[2
]}

Trivedi, Garima ^{[1
]}

Anand, Shashi ^{[1
]}

Kaur, Amanpreet ^{[1
]}

Gupta, Pawan ^{[1
]}

Jain, Amita ^{[2
]}

Sharma, Charu ^{[1
]}

机构：

[1] CSIR Inst Microbial Technol, Sect 39A, Chandigarh 160036, India

[2] King George Med Univ, Dept Microbiol, Lucknow 226003, Uttar Pradesh, India

来源：

TUBERCULOSIS | 2016年 / 96卷

关键词：

Molecular characterization; Aminoglycosides; MDR-TB; XDR-TB; RESAZURIN MICROTITER ASSAY; MOLECULAR CHARACTERIZATION; DRUG-RESISTANCE; TRANSCRIPTIONAL ACTIVATOR; CAPREOMYCIN RESISTANCE; ANTIBIOTIC-RESISTANCE; CROSS-RESISTANCE; AMIKACIN; REGION; WHIB7;

D O I：

10.1016/j.tube.2015.10.012

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Twenty-nine Kanamycin resistant clinical isolates of Mycobacterium tuberculosis from Northern India were screened to evaluate genetic mutations in rrs gene, eis gene with its promoter, and whiB7 gene along with its 5'UTR. 14 strains (-48.0%) collectively exhibited mutations in rrs, eis or whiB7 target regions. While the highest frequency of mutations was found in rrs gene, eis and whiB7 loci displayed novel mutations. The novel mutations displayed by eis and whiB7 loci were found to be associated specifically with the Kanamycin resistance as none of the twenty nine Kanamycin sensitive strains harbor them. The inclusion of novel mutations of eis and whiB7 loci will be useful in improving the specificity of future diagnostics. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：96 / 101

页数：6

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