Studies on seed-borne mycoflora and aflatoxin B1 contaminations in food based seed samples: Molecular detection of mycotoxigenic Aspergillus flavus and their management

In the present study the mould incidence, ergosterol and aflatoxinB(1) (AFB(1)) contaminations were evaluated in different food based seed samples viz., chickpea, covvpea, green gram, groundnut, Indian bean, maize, sorghum, soya bean and sunflower collected from different agro-climatic regions of Karnataka (India). The agar plate and standard blotter methods were employed for determination of the fungal incidence, and the ergosterol and AFB1 contents were estimated qualitatively and quantitatively by TLC and spectrophotometric methods. For detection of aflatoxigenic isolates of A. flail's, the target gene specific primer aflR-F and atIR-R were used in PCR amplification. The antifungal and antiaflatoxigenic activities of some selected edible vegetable extracts were evaluated by measuring mycelial dry weight and AM, content. The results revealed that 15 diverse fungal species belonging to 11 genera were observed. Among the seed samples analyzed, the highest fungal incidence, ergosterol and AFB(1) were observed in sorghum samples followed by maize and chickpea. The PCR amplification showed positive results only for aflatoxigenic isolates of A. flavux and no amplification was observed in non-aflatoxigenic isolates and A. flavus isolate-2 produced highest AFB,, showed 99% similarity with an authenticated aflatoxigenic A. flavus isolate embIFN39816 1.1. The aqueous extract of Amorphophallus compottulcitus showed highest antifungal and antiallatoxigenic activities. The results confirmed that the ergosterol and AFBI contents were correlated with the percent mould incidences. (C) All Rights Reserved