Use of an alkaline phosphatase-conjugated oligonucleotide probe for the gene encoding the bundle-forming pilus of enteropathogenic Escherichia coli

被引：6
|
作者：
Nagayama, K [1 ]
Bi, Z [1 ]
Oguchi, T [1 ]
Takarada, Y [1 ]
Shibata, S [1 ]
Honda, T [1 ]
机构：
[1] TOYOBO RES CTR,OTSU,SHIGA 52002,JAPAN
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 1996年 / 34卷 / 11期
关键词：
D O I：
10.1128/JCM.34.11.2819-2821.1996
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
An alkaline phosphatase-conjugated 29-base oligonucleotide probe was developed to detect the gene encoding the bundle-forming pilus of enteropathogenic Escherichia coli. The sensitivity and specificity of the probe versus the results of localized adherence in the HEp-2 cell assay and fluorescent actin staining assay positivity were 95.7 and 100%, respectively.
引用
收藏
页码：2819 / 2821
页数：3
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