Use of an alkaline phosphatase-conjugated oligonucleotide probe for the gene encoding the bundle-forming pilus of enteropathogenic Escherichia coli

被引：6

作者：

Nagayama, K ^{[1
]}

Bi, Z ^{[1
]}

Oguchi, T ^{[1
]}

Takarada, Y ^{[1
]}

Shibata, S ^{[1
]}

Honda, T ^{[1
]}

机构：

[1] TOYOBO RES CTR,OTSU,SHIGA 52002,JAPAN

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1996年 / 34卷 / 11期

关键词：

D O I：

10.1128/JCM.34.11.2819-2821.1996

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

An alkaline phosphatase-conjugated 29-base oligonucleotide probe was developed to detect the gene encoding the bundle-forming pilus of enteropathogenic Escherichia coli. The sensitivity and specificity of the probe versus the results of localized adherence in the HEp-2 cell assay and fluorescent actin staining assay positivity were 95.7 and 100%, respectively.

引用

页码：2819 / 2821

页数：3

共 50 条

[31] The type IV bundle-forming pilus of enteropathogenic Escherichia coli undergoes dramatic alterations in structure associated with bacterial adherence, aggregation and dispersal
Knutton, S
Shaw, RK
Anantha, RP
Donnenberg, MS
Zorgani, AA
MOLECULAR MICROBIOLOGY, 1999, 33 (03) : 499 - 509
[32] REDUCED ADHESION TO HEP-2 CELLS OF ENTEROPATHOGENIC ESCHERICHIA-COLI (EPEC) GROWN TO PROMOTE BUNDLE-FORMING PILUS EXPRESSION
SMITH, CJ
MACK, DR
PEDIATRIC RESEARCH, 1994, 35 (04) : A136 - A136
[33] Prevalent transfer of human colostral IgA antibody activity for the enteropathogenic Escherichia coli bundle-forming pilus structural repeating subunit A in neonates
Fernandes, RCDC
Flores, VMQ
Medina-Acosta, E
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2002, 44 (04) : 331 - 336
[34] CLONING AND CHARACTERIZATION OF THE BUNDLE-FORMING PILIN GENE OF ENTEROPATHOGENIC ESCHERICHIA-COLI AND ITS DISTRIBUTION IN SALMONELLA SEROTYPES
SOHEL, I
PUENTE, JL
MURRAY, WJ
VUOPIOVARKILA, J
SCHOOLNIK, GK
MOLECULAR MICROBIOLOGY, 1993, 7 (04) : 563 - 575
[35] COMPARISON OF ALKALINE PHOSPHATASE-CONJUGATED OLIGONUCLEOTIDE DNA PROBE WITH THE SERENY TEST FOR IDENTIFICATION OF SHIGELLA STRAINS
PANDA, CS
RILEY, LW
KUMARI, SN
KHANNA, KK
PRAKASH, K
JOURNAL OF CLINICAL MICROBIOLOGY, 1990, 28 (09) : 2122 - 2124
[36] BfpB, an outer membrane lipoprotein required for the biogenesis of bundle-forming pili in enteropathogenic Escherichia coli
Ramer, SW
Bieber, D
Schoolnik, GK
JOURNAL OF BACTERIOLOGY, 1996, 178 (22) : 6555 - 6563
[37] IDENTIFICATION OF ROTAVIRUSES BY DOT-BLOT HYBRIDIZATION USING AN ALKALINE PHOSPHATASE-CONJUGATED SYNTHETIC OLIGONUCLEOTIDE PROBE
YAMAKAWA, K
OYAMADA, H
NAKAGOMI, O
MOLECULAR AND CELLULAR PROBES, 1989, 3 (04) : 397 - 401
[38] Induction of epithelial cell death including apoptosis by enteropathogenic Escherichia coli expressing bundle-forming pili
Abul-Milh, M
Wu, Y
Lau, B
Lingwood, CA
Foster, DB
INFECTION AND IMMUNITY, 2001, 69 (12) : 7356 - 7364
[39] Role of intimin and bundle-forming pili in enteropathogenic Escherichia coli adhesion to pediatric intestinal tissue in vitro
Hicks, S
Frankel, G
Kaper, JB
Dougan, G
Phillips, AD
INFECTION AND IMMUNITY, 1998, 66 (04) : 1570 - 1578
[40] The Cpx envelope stress response affects expression of the type IV bundle-forming pili of enteropathogenic Escherichia coli
Nevesinjac, AZ
Raivio, TL
JOURNAL OF BACTERIOLOGY, 2005, 187 (02) : 672 - 686

← 1 2 3 4 5 →