Skin integrated with perfusable vascular channels on a chip

被引：215

作者：

Mori, Nobuhito ^{[1
]}

Morimoto, Yuya ^{[1
]}

Takeuchi, Shoji ^{[1
,2
]}

机构：

[1] Univ Tokyo, IIS, Ctr Int Res Integrat Biomed Syst CIBiS, Meguro Ku, 4-6-1 Komaba, Tokyo 1538505, Japan

[2] JST, Exploratory Res Adv Technol ERATO, Takeuchi Biohybrid Innovat Project, KOL,Meguro Ku, Room M202,4-6-1,Komaba, Tokyo 1538904, Japan

来源：

BIOMATERIALS | 2017年 / 116卷

基金：

日本学术振兴会;

关键词：

Skin-equivalent; Skin model; Vascular channel; Perfusion; Blood vessel; Percutaneous absorption; IN-VITRO; TISSUE; CELL; VIVO; EQUIVALENT; PARYLENE; DERMIS; MODELS;

D O I：

10.1016/j.biomaterials.2016.11.031

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

This paper describes a method for fabricating perfusable vascular channels coated with endothelial cells within a cultured skin-equivalent by fixing it to a culture device connected to an external pump and tubes. A histological analysis showed that vascular channels were constructed in the skin-equivalent, which showed a conventional dermal/epidermal morphology, and the endothelial cells formed tight junctions on the vascular channel wall. The barrier function of the skin-equivalent was also confirmed. Cell distribution analysis indicated that the vascular channels supplied nutrition to the skin-equivalent. Moreover, the feasibility of a skin-equivalent containing vascular channels as a model for studying vascular absorption was demonstrated by measuring test molecule permeation from the epidermal layer into the vascular channels. The results suggested that this skin-equivalent can be used for skin-on-a-chip applications including drug development, cosmetics testing, and studying skin biology. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：48 / 56

页数：9

共 30 条

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