Reduced Plate Height of 1.65 on a 20 x 3 mm Column Packed with 1.8 μm Particles in Supercritical Fluid Chromatography (SFC)

被引：7

作者：

Berger, Terry A. ^{[1
]}

机构：

[1] SFC Solut Inc, Englewood, FL 34224 USA

来源：

CHROMATOGRAPHIA | 2019年 / 82卷 / 06期

关键词：

Supercritical fluid chromatography (SFC); 3; x; 20; mm; 1.8 mu m column; Reduced plate height of 1.65; 75 mu m tubing; Very high pressure; PERFORMANCE; EFFICIENCY;

D O I：

10.1007/s10337-019-03722-z

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The plumbing of a commercial supercritical fluid chromatograph was modified by replacing all the connector tubing with the shortest possible lengths (65 cm) of 75 mu m ID tubes. Heat exchangers controlling column temperatures and column outlet temperature were bypassed. The UV detector flow cell was replaced with an experimental cell with a 75 mu m inlet tube, and a 2 mu L internal volume. All these steps were taken to minimize extra-column dispersion. The extra-column dispersion was decreased from approximate to 80 mu L-2 in the commercial instrument to roughly 2 mu L-2 with the modifications. Reduced plate heights as low as 1.65 were obtained using a 20 x 3 mm column packed with 1.8 mu m particles. This is a remarkably low value for such a short column. The optimum flow was approximate to 2.5 mL min(-1). The system pressure drop at optimum was 220 bar (320 bar pump pressure), but increased rapidly with flow rate, to 450 bar (550 at pump) at 3.5 mL min(-1).

引用

页码：971 / 974

页数：4

共 13 条

[11] Comparison of ultra-high performance supercritical fluid chromatography and ultra-high performance liquid chromatography for the analysis of pharmaceutical compounds [J].