ExoT of cytotoxic Pseudomonas aeruginosa prevents uptake by corneal epithelial cells

被引：86
作者：
Cowell, BA
Chen, DY
Frank, DW
Vallis, AJ
Fleiszig, SMJ
机构：
[1] Univ Calif Berkeley, Sch Optometry, Berkeley, CA 94720 USA
[2] Med Coll Wisconsin, Dept Microbiol & Mol Genet, Milwaukee, WI 53226 USA
来源：
INFECTION AND IMMUNITY | 2000年 / 68卷 / 01期
关键词：
D O I：
10.1128/IAI.68.1.403-406.2000
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
The presence of invasion-inhibitory activity that is regulated by the transcriptional activator ExsA of cytotoxic Pseudomonas aeruginosa has previously been proposed. The results of this study show that both ExoT and ExoS, known type III secreted effector proteins of P. aeruginosa that are regulated by ExsA, possess this activity. Invasion was reduced 94.4% by ExoT and 96.0% by ExoS. Invasion-inhibitory activity is not linked to ADP-ribosylation activity, at least for ExoS, since a noncatalytic mutant also inhibits uptake by an epithelial cell line (invasion was reduced 96.0% by ExoSE381A).
引用
收藏
页码：403 / 406
页数：4
相关论文
共 20 条
[11]   PROTEIN TYROSINE PHOSPHATASE-ACTIVITY OF AN ESSENTIAL VIRULENCE DETERMINANT IN YERSINIA [J].
GUAN, KL ;
DIXON, JE .
SCIENCE, 1990, 249 (4968) :553-556
[12]   Defects in type III secretion correlate with internalization of Pseudomonas aeruginosa by epithelial cells [J].
Hauser, AR ;
Fleiszig, S ;
Kang, PJ ;
Mostov, K ;
Engel, JN .
INFECTION AND IMMUNITY, 1998, 66 (04) :1413-1420
[13]  
JUMBLATT MM, 1983, INVEST OPHTH VIS SCI, V24, P1139
[14]   INHIBITION OF PHAGOCYTOSIS IN YERSINIA-PSEUDOTUBERCULOSIS - A VIRULENCE PLASMID-ENCODED ABILITY INVOLVING THE YOP2B PROTEIN [J].
ROSQVIST, R ;
BOLIN, I ;
WOLFWATZ, H .
INFECTION AND IMMUNITY, 1988, 56 (08) :2139-2143
[15]   THE CYTOTOXIC PROTEIN YOPE OF YERSINIA OBSTRUCTS THE PRIMARY HOST DEFENSE [J].
ROSQVIST, R ;
FORSBERG, A ;
RIMPILAINEN, M ;
BERGMAN, T ;
WOLFWATZ, H .
MOLECULAR MICROBIOLOGY, 1990, 4 (04) :657-667
[16]   Regulation of ExoS production and secretion by Pseudomonas aeruginosa in response to tissue culture conditions [J].
Vallis, AJ ;
Yahr, TL ;
Barbieri, JT ;
Frank, DW .
INFECTION AND IMMUNITY, 1999, 67 (02) :914-920
[17]  
Vallis AJ, 1999, INFECT IMMUN, V67, P2040
[18]   Genetic relationship between the 53- and 49-kilodalton forms of exoenzyme S from Pseudomonas aeruginosa [J].
Yahr, TL ;
Barbieri, JT ;
Frank, DW .
JOURNAL OF BACTERIOLOGY, 1996, 178 (05) :1412-1419
[19]   ExoY, an adenylate cyclase secreted by the Pseudomonas aeruginosa type III system [J].
Yahr, TL ;
Vallis, AJ ;
Hancock, MK ;
Barbieri, JT ;
Frank, DW .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (23) :13899-13904
[20]   Identification of type III secreted products of the Pseudomonas aeruginosa exoenzyme S regulon [J].
Yahr, TL ;
MendeMueller, LM ;
Friese, MB ;
Frank, DW .
JOURNAL OF BACTERIOLOGY, 1997, 179 (22) :7165-7168
12