Development of a system for the detection of the inflammatory response induced by airborne fine particulate matter in rat tracheal epithelial cells

被引：3

作者：

Yamagishi, Nobuyuki ^{[1
]}

Yamaguchi, Tomoki ^{[1
]}

Kuga, Takahisa ^{[1
]}

Taniguchi, Masanari ^{[1
]}

Khan, Mohammad Shahriar ^{[2
]}

Matsumoto, Takahiro ^{[2
]}

Deguchi, Yuya ^{[3
]}

Nagaoka, Hiroaki ^{[3
]}

Wakabayashi, Keiji ^{[2
,4
]}

Watanabe, Tetsushi ^{[2
]}

机构：

[1] Setsunan Univ, Fac Pharmaceut Sci, Dept Analyt Biomol, 45-1 Nagaotoge Cho, Hirakata, Osaka 5730101, Japan

[2] Kyoto Pharmaceut Univ, Dept Publ Hlth, Yamashina Ku, 1 Misasagishichono Cho, Kyoto 6078412, Japan

[3] Nagasaki Int Univ, Fac Pharmaceut Sci, 2825-7 Huis 10 Bosch, Nagasaki 8593298, Japan

[4] Univ Shizuoka, Grad Div Nutr & Environm Sci, Suruga Ku, 52-1 Yada, Shizuoka 4228526, Japan

来源：

TOXICOLOGY REPORTS | 2020年 / 7卷

关键词：

Airborne particulate matter; Endotoxin; Pro-inflammatory cytokines; Fine particle; Coarse particle; OXIDATIVE STRESS; AIR-POLLUTANTS; PARTICLE MASS; URBAN AIR; IN-VITRO; ENDOTOXIN; ASTHMA; EXPOSURE; DUST; HOSPITALIZATION;

D O I：

10.1016/j.toxrep.2020.06.011

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

Exposure to airborne particulate matter (PM) is related to the increased risk of several diseases, including chronic and allergic rhinitis. We have previously shown that atmospheric endotoxin level was positively associated with the number of emergency department visits for asthma even after adjusting for meteorological factors, suggestive of the significant association between atmospheric endotoxin level and asthma exacerbation. Whether atmospheric endotoxin level is related to inflammatory response induction is, however, unclear. Here, we established stable cell lines to determine the promoter activity of the genes encoding pro-inflammatory cytokines such as tumor necrosis factor alpha, interleukin 6 (IL6), and IL33 by transfection of each reporter plasmid into rat tracheal epithelial EGV-4 T cells. These cells could measure the inflammatory response induced by endotoxin treatment more easily, rapidly, and sensitively than the conventional system using immunodetection assays. Furthermore, we revealed a relationship between atmospheric endotoxin level and inflammatory response induction. Thus, the system established herein may serve as a promising tool to monitor inflammatory response induced upon PM exposure.

引用

页码：900 / 908

页数：9

共 34 条

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