Use of ELISA for in vitro potency test of rabies vaccines for animal use

被引：39

作者：

Gamoh, K ^{[1
]}

Senda, M ^{[1
]}

Itoh, O ^{[1
]}

Muramatsu, M ^{[1
]}

Hirayama, N ^{[1
]}

Koike, R ^{[1
]}

Endoh, YS ^{[1
]}

Minamoto, N ^{[1
]}

机构：

[1] GIFU UNIV,FAC AGR,YANAGI 50111,JAPAN

来源：

BIOLOGICALS | 1996年 / 24卷 / 02期

关键词：

D O I：

10.1006/biol.1996.0012

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The use of ELISA for the potency test of inactivated rabies vaccines for animal use was evaluated. Sandwich ELISA was developed, using monoclonal antibodies which enabled to recognize the glycoprotein (G-protein) of rabies virus and showed a protective effect in mice. Soluble G-protein which displays an ELISA activity but a low immunogenicity was removed by gel filtration before ELISA was carried out. The ELISA titres of the first fraction of vaccines separated by gel filtration were correlated with the neutralizing antibody titres of vaccinated guinea-pigs. Our results suggest that the ELISA titres of the first fraction of vaccines separated by gel filtration reflected the protective G-protein contents for the in vitro potency test of rabies vaccines compared with the current in vivo test. (C) 1996 The International Association of Biological Standardization

引用

页码：95 / 101

页数：7

共 20 条

[1]

ADMOWICZ P, 1984, DEV BIOL STAND, V55, P191

[2] NIH TEST, A PROBLEMATIC METHOD FOR TESTING POTENCY OF INACTIVATED RABIES VACCINE [J].