Determination of furosine in hydrolyzate of processed milk by HPLC using a narrow bore column and diode-array detector

被引：5
|
作者：
Nicoletti, I
Cogliandro, E
Corradini, C
Corradini, D
Pizzoferrato, L
机构：
[1] CNR, Ist Cromatog, Area Ric Roma, I-00016 Monterotondo, Rome, Italy
[2] Ist Nazl Nutr, I-00178 Rome, Italy
来源：
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2000年 / 23卷 / 05期
关键词：
D O I：
10.1081/JLC-100101484
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A simple, rapid, sensitive, reproducible, and accurate HPLC procedure employing a narrow bore reversed phase column and a diode-array detector is proposed for the determination of epsilon-N-2-furoylmethyl-L-lysine (furosine) in acid hydrolyzed of milk and powdered milk samples. After optimization of the variables involved, the method was characterized and validated in terms of selectivity, repeatability, sensitivity, and accuracy and applied to determine furosine content in hydrolyzed milk samples.
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页码：717 / 726
页数：10
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