Evaluation of genetic diversity in Chinese kale (Brassica oleracea L. var. alboglabra Bailey) by using rapid amplified polymorphic DNA and sequence-related amplified polymorphism markers

被引：4
作者：
Zhang, J. [1 ,2 ,3 ]
Zhang, L. G. [1 ,3 ]
机构：
[1] Northwest A&F Univ, Coll Hort, Yangling, Shaanxi, Peoples R China
[2] Shanxi Agr Univ, Coll Hort, Taigu, Shaanxi, Peoples R China
[3] State Key Lab Crop Stress Biol Arid Areas, Yangling, Shaanxi, Peoples R China
来源：
GENETICS AND MOLECULAR RESEARCH | 2014年 / 13卷 / 02期
关键词：
Chinese kale; Genetic diversity; RAPD markers; SRAP markers; CAULIFLOWER CULTIVARS; GERMPLASM; SRAP;
D O I：
10.4238/2014.February.14.10
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Chinese kale is an original Chinese vegetable of the Cruciferae family. To select suitable parents for hybrid breeding, we thoroughly analyzed the genetic diversity of Chinese kale. Random amplified polymorphic DNA (RAPD) and sequence-related amplified polymorphism (SRAP) molecular markers were used to evaluate the genetic diversity across 21 Chinese kale accessions from AVRDC and Guangzhou in China. A total of 104 bands were detected by 11 RAPD primers, of which 66 (63.5%) were polymorphic, and 229 polymorphic bands (68.4%) were observed in 335 bands amplified by 17 SRAP primer combinations. The dendrogram showed the grouping of the 21 accessions into 4 main clusters based on RAPD data, and into 6 clusters based on SRAP and combined data (RAPD + SRAP). The clustering of accessions based on SRAP data was consistent with petal colors. The Mantel test indicated a poor fit for the RAPD and SRAP data (r = 0.16). These results have an important implication for Chinese kale germplasm characterization and improvement.
引用
收藏
页码：3567 / 3576
页数：10
相关论文
共 26 条
[11]   Genetic diversity in four cabbage populations based on random amplified polymorphic DNA markers [J].
Koutita, O ;
Tertivanidis, K ;
Koutsos, TV ;
Koutsika-Sotiriou, M ;
Skaracis, GN .
JOURNAL OF AGRICULTURAL SCIENCE, 2005, 143 :377-384
[12]   STUDY OF BIODIESEL PRODUCTION FROM OILSEED PLANTS I. EVALUATION OF THE GENETIC SIMILARITY OF SOME Brassica Napus CULTIVARS USING RAPD MARKERS [J].
Leonte, Constantin ;
Arsene, Madalina Cristina Burlacu ;
Simioniuc, Danut ;
Vatavu, Roxana ;
Calistru, Anca Elena .
ENVIRONMENTAL ENGINEERING AND MANAGEMENT JOURNAL, 2010, 9 (09) :1305-1310
[13]   Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction:: its application to mapping and gene tagging in Brassica [J].
Li, G ;
Quiros, CF .
THEORETICAL AND APPLIED GENETICS, 2001, 103 (2-3) :455-461
[14]   Cultivar identification and genetic diversity analysis of broccoli and its related species with RAPD and ISSR markers [J].
Lu, Xinjuan ;
Liu, Liwang ;
Gong, Yiqin ;
Zhao, Liping ;
Song, Xianyong ;
Zhu, Xianwen .
SCIENTIA HORTICULTURAE, 2009, 122 (04) :645-648
[15]  
MANTEL N, 1967, CANCER RES, V27, P209
[16]  
Matsui T, 2002, J JPN SOC HORTIC SCI, V71, P499, DOI 10.2503/jjshs.71.499
[17]  
Okumus A, 2007, Genetika, V43, P516
[18]   Genetic diversity in mustard (Brassica juncea L.) germplasm from Pakistan as determined by RAPDs [J].
Rabbani, MA ;
Iwabuchi, A ;
Murakami, Y ;
Suzuki, T ;
TTakayanagi, K .
EUPHYTICA, 1998, 103 (02) :235-242
[19]  
RHOLF FJ, 2000, NTSYS PC NUMERICAL T
[20]   Genetic diversity of oilseed Brassica napus inbred lines based on sequence-related amplified polymorphism and its relation to hybrid performance [J].
Riaz, A ;
Li, G ;
Quresh, Z ;
Swati, MS ;
Quiros, CF .
PLANT BREEDING, 2001, 120 (05) :411-415
123